Re: [Histonet] DNA streaming out of nuclei in frozen sections?

2009-01-04 Thread Rene J Buesa
, Pritchard, Michele wrote: From: Pritchard, Michele Subject: [Histonet] DNA streaming out of nuclei in frozen sections? To: histonet@lists.utsouthwestern.edu Date: Sunday, January 4, 2009, 1:37 PM Hello: I have recently begun to use frozen sections to localize an antigen found in mouse liver

[Histonet] DNA streaming out of nuclei in frozen sections?

2009-01-04 Thread Pritchard, Michele
Hello: I have recently begun to use frozen sections to localize an antigen found in mouse liver tissue. My previous experience has been almost exclusively with staining of formalin-fixed, paraffin-embedded tissues. I have done two runs of this new staining protocol and noticed an odd artifa