In looking through old procedures, I have found several different 'solutions'
that have been used for fixation and decalcification of bone (particularly bone
marrow cores). I would very much appreciate hearing what people are using
today for optimal fixation and decalcification of bone for
...@lists.utsouthwestern.edu] on behalf of Jeanne Clark
[paintedsplas...@yahoo.com]
Sent: Wednesday, July 25, 2012 10:14 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Decalcifying bone
In looking through old procedures, I have found several different 'solutions'
that have been used for fixation
Bone marrow biopsy specimens can be decalcified with a variety of
commercial and home-brewed decalcifiers. Always you have to make sure
you don't leave the specimen in the decalcifier for too long.
All decalcifiers leach out iron (hemosiderin). The iron stain should
be done on the undecalcified
, Kerry
Sent: Friday, January 14, 2011 4:32 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] decalcifying bone marrows after processing
I was wondering if anyone has any experience with, or is it even possible to,
decalcify bone marrows after they are processed. Our pathologist would
I was wondering if anyone has any experience with, or is it even possible to,
decalcify bone marrows after they are processed. Our pathologist would like to
be able to process bone marrows the same day we receive them, but most of the
time there just isn't enough time to allow for proper
of the ignorance of most pathologists about tissue
processing things.
René J.
--- On Fri, 1/14/11, Powers, Kerry powe...@ccmhonline.com wrote:
From: Powers, Kerry powe...@ccmhonline.com
Subject: [Histonet] decalcifying bone marrows after processing
To: histonet@lists.utsouthwestern.edu
Date: Friday