The Paths are noticing a halo efftect around the keratinocytes. This is something new. It appears to be happening during staining. We haven't changed anything and we are making sure that all the reagents are fresh and full. Thoughts? Thanks, Kristen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet