RE: [Histonet] EIER before HIER??

2008-11-03 Thread Sebree Linda A.
53792 (608)265-6596 -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Woodward, Denise Sent: Monday, November 03, 2008 3:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] EIER before HIER?? Hi folks, I have a new antibody that seems to need both Pro

[Histonet] EIER before HIER??

2008-11-03 Thread Woodward, Denise
Hi folks, I have a new antibody that seems to need both Protease digestion and Citrate buffer antigen retrieval. Does anyone have any experience with this double whammy approach and know if it works best to do EIER before HIER or HIER before EIER. Perhaps it doesn't matter which is first? Any