Fortunate enough to purchase and use several TEM and SEM's over the ye= ars, I always found Zeiss to have the best prices and products for TEM and = Hitachi for the SEM's. Last one I bought was 200K plus. Good Luck and= let me know if you know of anyone looking for an EM tech in the dc area.
Nick(Rocky) Madary, HT/HTL(ASCP)QIHC Joni Madary, PhD(in life)May 2= 4, 2011 12:01:20 PM, histonet@lists.utsouthwestern.edu wrote: Send Histonet= mailing list submissions to histonet@lists.utsouthwestern.edu To= subscribe or unsubscribe via the World Wide Web, visit http://lists.uts= outhwestern.edu/mailman/listinfo/histonet or, via email, send a message = with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lis= ts.utsouthwestern.edu When replying, please edit your Subject line s= o it is more specific than "Re: Contents of Histonet digest..." <= BR>Today's Topics: 1. RE: p63 (McMahon, Loralee A) 2. Re: Histone= t Digest, Vol 90, Issue 28 (bonesli...@aol.com) 3. RE: p63 (Bell, Lynne) 4. Re: Freezing bath solutions (Robert Richmond) 5. Jones stain boo = boo (Norton, Sally) 6. Re: Re: Freezing bath solutions (mwhite@mcleodhea= lth.org) 7. IHC Control Tissues (Goins, Tresa) 8. FISH (wassan alkadh= umi) 9. AW: [Histonet] FISH (Gudrun Lang) 10. Las Vegas Position (Hal= e, Meredith) 11. RE: p63 (Dessoye, Michael J) 12. Thank you (Margaret= Blount) 13. Re: Re: Freezing bath solutions (abright@brightinstruments. com) 14. decal (Cynthia Pyse) 15. Re: decal (Rene J Buesa) 16. Pro= cessor power supply Back-up (kristen arvidson) 17. Coronavirus antibody = (Chakib Boussahmain) 18. Pathology Transmission Electron Micrscope (Vick= roy, Jim) ------------------------------------------------------ ---------------- Message: 1 Date: Mon, 23 May 2011 13:33:27 -0400 From: "McMahon, Loralee A" Subj= ect: [Histonet] RE: p63 To: Joanne Clark , "histonet= @lists.utsouthwestern.edu" Messag= e-ID: Content-Type: text/plain; charset="us-ascii" Bioca= re Medical Loralee McMahon, HTL (ASCP) Immunohistochemistry Super= visor Strong Memorial Hospital Department of Surgical Pathology (5= 85) 275-7210 ________________________________________ From: histonet-= boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu= ] On Behalf Of Joanne Clark [jcl...@pcnm.com] Sent: Monday, May 23, 2011= 12:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] p= 63 I need to find a source for p63 antibody. Where does everyone pur chase theirs from? Joanne Clark,HT Pathology Consultants of New M= exico _______________________________________________ Histonet ma= iling list Histonet@lists.utsouthwestern.edu http://lists.utsouthwest= ern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Mon, 23 May 2011 13:41:36 -0400 (EDT) From: = bonesli...@aol.com Subject: [Histonet] Re: Histonet Digest, Vol 90, Issu= e 28 To: histonet@lists.utsouthwestern.edu Message-ID: <8CDE787CC3 64fab-17a0-7f...@webmail-d058.sysops.aol.com> Content-Type: text/plai= n; charset="utf-8" Dear Miss Margaret Blount: Congratulatio= ns on your retirement!! May you enjoy good health and many years doing the = things you WANT to do!! God Bless! -----Origi= nal Message----- From: histonet-request To: histonet Sent: Mo= n, May 23, 2011 1:00 pm Subject: Histonet Digest, Vol 90, Issue 28 Send Histonet mailing list submissions to histonet@lists.utsouthwe= stern.edu To subscribe or unsubscribe via the World Wide Web, visit h= ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet r, via email, s= end a message with subject or body 'help' to histonet-requ...@lists.utso= uthwestern.edu You can reach the person managing the list at histonet= -ow...@lists.utsouthwestern.edu When replying, please edit your Subject = line so it is more specific han "Re: Contents of Histonet digest..." oday's Topics: 1. Immuno controls (Carrie Disbrow) 2. This is goo= dbye (Margaret Blount) 3. In vitro mouse embryo culture (Carmen Maria Ga= rcia Pascual) 4. Re: Immuno controls (Rene J Buesa) 5. RE: Histonet D= igest, Vol 90, Issue 27 (Barone, Carol ) 6. RE: Histonet Digest, Vol 90,= Issue 27 (Barone, Carol ) 7. Freezing bath solutions (Scott, Allison D) 8. p63 (Joanne Clark) ------------------------------------------ --------------------------- Message: 1 ate: Sun, 22 May 2011 22:00:05= -0400 rom: Carrie Disbrow ubject: [Histonet] Immu= no controls o: essage-ID: ontent-Type: text/plain; charset= ="iso-8859-1" i all, fter consideration I disagree with the= consensus and also agree with Curt's athologist. More supplies and lab= or will be involved as cutting more controls ill be neccessay to meet t= he requests of the pathologist. The patient tissue is ut on two control= s slides. One slide is used as the negative control and has oth the pat= ient tissue and the known positive control which should both be egative= after the run. The other slide with the known positive control and the atient tissue in question is also stained with the positive control staini= ng ositive. hank you! ------------------------------ Messa= ge: 2 ate: Mon, 23 May 2011 09:04:54 +0100 rom: "Margaret Blount" ubject: [Histonet] This is goodbye o: essage-ID: ontent-Type: text/plain; charset= ="US-ASCII" To everyone in histoland, This is my last full week= at work. From next Tuesday I will be retiring rom my job at Cambridge U= niversity. I have thoroughly enjoyed my chosen rofession as a research t= echnician specialising in histology. I realise hat because I have always= worked in research my experience is somewhat imited: I haven't been ask= ed for the same range of techniques that you ind in a routine clinical l= ab. However, I have enjoyed my work and have earnt and devised some tech= niques for a range of interesting samples. ow it's time to learn somethi= ng new! I hope to spend more time with my lace pillow and learn to = do it roperly, maybe learn to do Northamptonshire lace which I believe i s a ariation on Bucks point. (I come originally from Northants) I have h= ad go at Bedfordshire and will learn more, it is very pretty. I also pla= n o travel; my next trip is to New Zealand in November. I won't = go on. I just want to say this: You are all great guys and irls; you sha= re your experience and friendship with great generosity, hich I have alw= ays felt is what a scientist should do. Thank you for our comradeship an= d help over the years. Maybe I'll get to meet some of ou by chance durin= g my travels. Best wishes to you all and a great big Thank You, <= BR>From Margaret Miss Margaret Blount Histology Manager Metabolic Research Laboratories Level 4 Institute of Metabolic Science<= BR>Box 289, Addenbrooke's Hospital Hills Road, Cambridge, CB2 0QQ Tel 01223 769061/336079 ------------------------------ Messa= ge: 3 ate: Mon, 23 May 2011 10:35:20 +0200 (CEST) rom: "Carmen Maria = Garcia Pascual" ubject: [Histonet] In vitro mouse= embryo culture o: histonet@lists.utsouthwestern.edu essage-ID: <6= 395165382carma...@uv.es> ontent-Type: text/plain; charset="ISO-8859= -1" Good Moorning to everyone: I am Carmen Garcia a PhD student f= rom Valencia, we are know interesting n make in vitro culture of mouse = embryos to do toxicity tests and in ur lab we have not experience. some= one can give me some advise? or ecommend me some bibliography? ow lo= ng can I have the embryos in culture? hank you so much!!! Best regard= s, armen ------------------------------ Message: 4 ate:= Mon, 23 May 2011 07:48:50 -0700 (PDT) rom: Rene J Buesa ubject: Re: [Histonet] Immuno controls o: Carrie Disbrow , "histonet@lists.utsouthwestern.edu" essage-ID: <87688.31423...@web65714.mail.ac4.yahoo.co= m> ontent-Type: text/plain; charset=iso-8859-1 Also Sprach Zarat= hustra!!!! en� J. From: Carrie Disbrow o: histonet@lists.utsouthwestern.edu ent: Sunday, May 22, 2011 = 10:00 PM ubject: [Histonet] Immuno controls i all, fter co= nsideration I disagree with the consensus and also agree with Curt's at= hologist. More supplies and labor will be� involved as�= �¿½ cutting more controls ill be neccessay to meet the requ= ests of the pathologist. The patient tissue is ut on two controls slide= s. One slide is used as the negative control and has oth the patient ti= ssue and the known positive control which should both be egative after = the run. The other slide with the known positive control and the atient= tissue in question is also stained with the positive control staining = ositive. hank you! ��ï¿= ½ ��� ï¿= ½ï¿½ï¿½ � ï¿ ½ï¿½ï¿½ �ï¿= ½ï¿½ � _______________________________ ________________ istonet mailing list isto...@lists.utsouthwestern.ed= u ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------= -------------------- Message: 5 ate: Mon, 23 May 2011 11:02:16 -0400<= BR>rom: "Barone, Carol " ubject: [Histonet] RE: Hi= stonet Digest, Vol 90, Issue 27 o: essage-ID: ontent-Type: text/plain; charset="us-ascii" -----Original Me= ssage----- rom: histonet-boun...@lists.utsouthwestern.edu mailto:hist= onet-boun...@lists.utsouthwestern.edu] On Behalf Of istonet-request@list= s.utsouthwestern.edu ent: Sunday, May 22, 2011 1:01 PM o: histonet@li= sts.utsouthwestern.edu ubject: Histonet Digest, Vol 90, Issue 27 Send= Histonet mailing list submissions to To subscribe or unsubscribe vi= a the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/list= info/histonet r, via email, send a message with subject or body 'help' t= o histonet-requ...@lists.utsouthwestern.edu You can reach the person = managing the list at histonet-ow...@lists.utsouthwestern.edu When rep= lying, please edit your Subject line so it is more specific than Re: Con= tents of Histonet digest..." oday's Topics: 1. to Rene J Buesa (M= axim Peshkov) ------------------------------------------------------ --------------- Message: 1 ate: Sun, 22 May 2011 13:43:07 +0400 ro= m: Maxim Peshkov ubject: [Histonet] to Rene J Buesao: Rene J Buesa c: histonet@lists.utsouthwestern.e= du essage-ID: <1585823684.20110522134...@mail.ru> ontent-Type: = text/plain; charset=windows-1251 I am sorry, that uses histonet for pe= rsonal goals. Dear Rene! ast 3 my answers for you are returned back t= o me. our e-mails I got successfully. upport service of mail.ru wrote= that problem is at yahoo.com server. lease check your postmaster servic= e. Here is my post-address: axim Peshkov, l. Mikhailovskaya, 90-A, 47909, aganrog, ussia. Sincerely, axim. mailto:Maxim_71@= mail.ru ----------------------------- _______________________= ________________________ istonet mailing list istonet@lists.utsouthwe= stern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet En= d of Histonet Digest, Vol 90, Issue 27 *********************************= ****** ------------------------------ Message: 6 ate: Mon, 23 = May 2011 11:15:34 -0400 rom: "Barone, Carol " u= bject: [Histonet] RE: Histonet Digest, Vol 90, Issue 27 o: essage-ID: ontent-Type: text/plain; charset="us-ascii" Histonetters: Needed to stain xenograft with "Bunny"-IgG-direct, thatad already been in formailin two weeks (was FFPE). Normally would do n= a frozen 1:40? ....We have background everywhwere on first un...suggest= ions? Neg is also shining??? Crazy researcher's! Got to ove them...when = will they learn to ask first? We are diluting primary in plan two, and w= ill stain over-night at 4 egrees....but, this thing is lit-up like the S= UN! was expecting low signal? Go figure? ----Original Message---= -- rom: histonet-boun...@lists.utsouthwestern.edu mailto:histonet-bou= n...@lists.utsouthwestern.edu] On Behalf Of istonet-request@lists.utsout= hwestern.edu ent: Sunday, May 22, 2011 1:01 PM o: histo...@lists.utso= uthwestern.edu ubject: Histonet Digest, Vol 90, Issue 27 Send Histone= t mailing list submissions to histonet@lists.utsouthwestern.edu To su= bscribe or unsubscribe via the World Wide Web, visit http://lists.utsout= hwestern.edu/mailman/listinfo/histonet r, via email, send a message with= subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu = You can reach the person managing the list at histonet-owner@lists.utsou= thwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest..." oday's Topics: 1. to Rene J Buesa (Maxim Peshkov) ----------------------------- ---------------------------------------- Message: 1 ate: Sun, 22 May = 2011 13:43:07 +0400 rom: Maxim Peshkov ubject: [Hi= stonet] to Rene J Buesa o: Rene J Buesa c: histon= e...@lists.utsouthwestern.edu essage-ID: <1585823684.20110522134307@mai= l.ru> ontent-Type: text/plain; charset=windows-1251 I am sorry, = that uses histonet for personal goals. Dear Rene! ast 3 my answers fo= r you are returned back to me. our e-mails I got successfully. upport= service of mail.ru wrote that problem is at yahoo.com server. lease che= ck your postmaster service. Here is my post-address: axim Peshkov, l. Mikhailovskaya, 90-A, 47909, aganrog, ussia. Sincerely, = axim. mailto:maxim...@mail.ru -----------------------------______________________________________ _________ istonet mailing lististo...@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailma= n/listinfo/histonet End of Histonet Digest, Vol 90, Issue 27 ********= ******************************* ------------------------------ Me= ssage: 7 ate: Mon, 23 May 2011 11:22:24 -0500 rom: "Scott, Allison D" ubject: [Histonet] Freezing bath solutions o: essage-ID: <1872B4A455B= 7974391609ad8034c79fc026df...@lbexch01.hchd.local> ontent-Type: text/= plain; charset="us-ascii" Hello to all in histoland. What type of solu= tions are being used if any o freezze your chucks for frozen sections. O= ur sister hospital is sing methyl butane and there was a flash fire last= week. We use the ame solution. Any suggestions will be greatly apprecia= ted. Allison Scott HT(ASCP) istology Supervisor BJ Hospital ONF= IDENTIALITY NOTICE: f you have received this e-mail in error, please imm= ediately notify the ender by return e-mail and delete this e-mail and an= y attachments from our computer system. To the extent the informatio= n in this e-mail and any attachments contain rotected health informatio= n as defined by the Health Insurance Portability nd Accountability Act = of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 64; or Chapter 181,= Texas Health and Safety Code, it is confidential and/or rivileged. Thi= s e-mail may also be confidential and/or privileged under exas law. The= e-mail is for the use of only the individual or entity named bove. If = you are not the intended recipient, or any authorized epresentative of = the intended recipient, you are hereby notified that any eview, dissemi= nation or copying of this e-mail and its attachments is trictly prohibi= ted. ------------------------------ Message: 8 ate: Mon, 23 Ma= y 2011 16:31:08 +0000 rom: Joanne Clark ubject: [Hi= stonet] p63 o: "histonet@lists.utsouthwestern.edu" essage-ID: <0494A7D4E8CC254EA2FB81464982E3786DCA= 7...@s10maild001n1.sh10.lan> ontent-Type: text/plain; charset="us-asc= ii" I need to find a source for p63 antibody. Where does everyone purcha= se theirs rom? Joanne Clark,HT athology Consultants of New Mexico ------------------------------ _________________________________= ______________ istonet mailing list isto...@lists.utsouthwestern.edu< BR>ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histo= net Digest, Vol 90, Issue 28 * --------------------------= ---- Message: 3 Date: Mon, 23 May 2011 13:45:50 -0400 From: "B= ell, Lynne" Subject: [Histonet] RE: p63 To: 'Jo= anne Clark' , "histonet@lists.utsouthwestern.edu" Message-ID: Content-Type: text/plain; char= set="us-ascii" I believe the only place you can buy p63 is through= Biocare, www.biocare.net. Lynne Bell, HT (ASCP) Histology Te= am Leader 802-371-4923 ------------------------------ Message: 4 Date: Mon, 23 May 2011 14:12:37 -0400 From: Robert Ric= hmond Subject: [Histonet] Re: Freezing bath solut= ions To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset= =ISO-8859-1 Allison Scott after a flash fire with the stuff asks a= bout alternatives to 2-methylbutane for freezing specimens. There= is indeed a safe alternative, though I haven't had a chance to try it. = I've posted about it on Histonet more than once - here's a copy of an ea= rlier post of mine: 3M(tm) Novec(tm) Engineered Fluid HFE-7100 Th= is product belongs to a class of fluorocarbons called "segregated hydrof= luoroethers (HFE's)" According to various MSDS, HFE-7100 is methyl no= nafluoroisobutyl ether C4F9-O-CH3 It melts and freezes at -135 C, so = that it would remain liquid in the Histobath. (It would freeze solid in = liquid nitrogen, however.) It boils at 60 C., and is listed as non-flamm= able. It has almost no vapor pressure at freezing temperatures. It co= st around $230 a gallon a few years ago. Bob Richmond Samurai Pat= hologist Knoxville TN ------------------------------ <= BR>Message: 5 Date: Mon, 23 May 2011 11:28:28 -0700 From: "Norton, Sa= lly" Subject: [Histonet] Jones stain= boo boo To: Message-ID: <1= 6e0693c7018c245959ac729fe66ede52db...@s107.childrens.sea.kids> Conten= t-Type: text/plain; charset="us-ascii" I accidentally put my renal= slides in the Methanimine Silver (after Periodic Acid) before putting t= hem in the Thiosemicarbizine. Slides take on a yellow hue which makes th= e H&E counterstain muddy. Is there a way to correct this? Thank you, Sally Norton Histotech CONFIDENTIALITY NOTICE: This e-mail message, including any attach ments, is for the sole use of the intended recipient(s) and may contain con= fidential and privileged information protected by law. Any unauthorized rev= iew, use, disclosure or distribution is prohibited. If you are not the inte= nded recipient, please contact the sender by reply e-mail and destroy all c= opies of the original message. -----------------------------= - Message: 6 Date: Mon, 23 May 2011 14:46:29 -0400 From: mwhit= e...@mcleodhealth.org Subject: Re: [Histonet] Re: Freezing bath solutionsTo: Robert Richmond Cc: histonet@lists.utsouthw= estern.edu, histonet-boun...@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="us-ascii" Allison and = Dr. Richmond: We are using Novec 7000 successfully in our freezing b= ath and have been doing so for about 6 months with no major problems. It does have some annoying traits ; however the safety benefits outwei= gh those issues , in my opinion. The issues are: a little more time requ= ired for specimen to freeze, tissues tending to float in the solution, a= nd evaporation. I worried that the evaporation would be a big problem , = but it has not .My techs discovered through experimentation that by lowe= ring the tissue slowly into the fluid, it would stop the flotation probl= em. Overall, I would recommend it. 3M has been very helpful to us. ( I'm not being paid in any way to endorse this!!) See below for 3M contac= t information. Additionally, through earlier Histonet inquiries, = I was told about a freezing bath for those of you who still use the old = Histobath that is no longer made. If anyone wants information about it, = It's on www.ftssystems.com and the item is called a "Mult-Cool Bath". We don't have one , but it looks like it could be a suitable replacement.<= BR> Here's our purchasing contact person at 3M: Christina Cerecedes1-888-509-5224 Melanie S. White, MT(ASCP) Laboratory Superv= isor, Systems/Anatomic Pathology McLeod Regional Medical Center Flore= nce, SC (843) 777-2072 NOTICE: This e-mail message and al= l attachments transmitted with it may contain legally PRIVILEGED and CO= NFIDENTIAL information intended solely for the use of the addressee. If= the reader of this message is not the intended recipient, you are here= by notified that any reading, dissemination, distribution, copying, or = other use of this message or its attachments is strictly prohibited. If= you have received this message in error, please notify the sender = immediately and/or notify the postmaster (postmas...@mcleodhealth.org), and= delete this message and all copies and backups thereof. Thank You. ------------------------------ Message: 7 Date: Mon, = 23 May 2011 18:56:05 +0000 From: "Goins, Tresa" Subje= ct: [Histonet] IHC Control Tissues To: "histo...@lists.utsouthwestern.ed= u" Message-ID: Content-Type: text/pla= in; charset="us-ascii" If you haven't made up your mind yet on thi= s issue, here are a couple of references that may - or may not - help: <= BR>Veterinary Diagnostics: http://www.ncbi.nlm.nih.gov/sites/entrez? orig_db=PubMed&db=pubmed&cmd=Search&term=%22Journal%20o f%20veterinary%20diagnostic%20investigation%20%3A%20official%20publ ication% 20of%20the%20American%20Association%20of%20Veterinary%20Laboratory% 20Diagno sticians%2C%20Inc%22%5BJour%5D%20AND%2020%5Bvolume%5D%20AND%20393%5 Bpage%5D= %20AND%202008%5Bpdat%5D FDA F.20 Quality Control: http://www.fda.gov/downloads/MedicalDevices/DeviceRegulationandGuid ance/Gui= danceDocuments/ucm094015.pdf Tresa Goins Histopathology Super= visor Department of Livestock Bozeman, Montana -------= ----------------------- Message: 8 Date: Mon, 23 May 2011 12:23:5= 4 -0700 (PDT) From: wassan alkadhumi Subject:= [Histonet] FISH To: h n Message-= ID: <539226.53686...@web45202.mail.sp1.yahoo.com> Content-Type: te= xt/plain; charset=iso-8859-1 Dear members We jus= t started doing FISH using HER2 FISH pharma Dx Dako kit, Code number K5331 on malignant breast tissues. I was wondering what is the succe= ss ratio or percentage for say a one run?? my hospital is the firs= t in our region to start FISH and I am the first to run it. = I have no experience with the hybridizer but by reading the&= nbsp;hybridizer manual and the kit insert i discover it is user friendly. <= BR>I run the first batch (six slides only) yesterday. Continue an= d finish it today. only three of the slides worked. I will star= t a new batch next week and I appreciate any help you may offer to avoi= d inaccurate result. Thank you Wassan Histotec= hnician Shorsh General Hospital North of Iraq ----------------= -------------- Message: 9 Date: Mon, 23 May 2011 22:15:37 +0200From: "Gudrun Lang" Subject: AW: [Histonet] FISH T= o: "'wassan alkadhumi'" Cc: histonet@lists.utsou= thwestern.edu Message-ID: <00DDDF450F674127ACFF7D694679751A@dielangs.= at> Hi Wassan, you may depart the FISH procedure in a tissue-d= ependant first part and a probe-dependant second part. The first part= comprises the pretreatment of the tissue to retrieve and permeabilises = it. The amount of pretreatment is dependant on the fixation-duration of = the tissue. The longer the fixation - the higher the temperature of the buffer, the longer the incubation time in the buffer, the longer the inc ubation time in the protease. Troubleshooting in this part: To= o low temperature of some slides because of inconsistent temperature in = the waterbath. Tissueslides mounted on the glass-slides in different hei= ghts. Too short protease-incubation in relation to the fixation time. Us ually nuclei can be digested until you see the first tiny holes. Underdi gested nuclei are dense and look grey in the triplefilter. If the nuc= lei are overdigested, they get bigger holes and fuzzy borders. Different= staining results due to variing fixation-times. The second part= comprises the stringent wash. The temperature, buffer-quality, duration= are probe-depending and are usually provided in the kit-handbook. Troubleshooting second part: Too high temp leads to loosing the bound = probe. Too low temp leads to high background because of unspecifc bound probes. With your problem I would try to prolong the digestion in 10= min steps, until a good result is found. Hint: standardization of fi= xation is the crucial point. In our lab the tumor-block is immediately f= ixed in a cassette as a FISH-block. So it will be fixed very well and wi= thstands the harsh treatment. The disadvantage is stronger autofluoresce= nce. A further hint: be sure, that the gum around the coverslip is harde= ned before starting the hybridizer-protocol. 10 min drying time. = I hope this helps Gudrun Lang -----Ursprüngliche= Nachricht----- Von: histonet-boun...@lists.utsouthwestern.edu [mailt= o:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von wassan alkad= humi Gesendet: Montag, 23. Mai 2011 21:24 An: h n Betreff: [Histon= et] FISH Dear members We just started doing FISH u= sing HER2 FISH pharma Dx Dako kit, Code number K5331 on malignant bre= ast tissues. I was wondering what is the success ratio or percenta= ge for say a one run?? my hospital is the first in our region&nb= sp;to start FISH and I am the first to run it. I have no experien= ce with the hybridizer but by reading the hybridizer = manual and the kit insert i discover it is user friendly. I run the f= irst batch (six slides only) yesterday. Continue and finish = it today. only three of the slides worked. I will start a new batc= h next week and I appreciate any help you may offer to avoid inaccura= te result. Thank you Wassan Histotechnician Shorsh General Hospital North of Iraq ______________________________= _________________ Histonet mailing list Histonet@lists.utsouthwestern= .edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Mo= n, 23 May 2011 16:53:16 -0500 From: "Hale, Meredith" Subject: [Histonet] Las Vegas Position To: Message-ID: <6F33D8418806044682A391273399860F08374FCA@s -irv-ex301.PathologyPartners.intranet> Content-Type: text/plain; = charset="us-ascii" Great opportunity for a Histotechnician= in a brand new laboratory! We are a 5 physician gastroenterology practi= ce located in Las Vegas, NV looking for a certified HT or HTL. Candidate= must meet CLIA grossing requirements. The candidate will be responsible= for routine histology duties. This is a part-time position that offers = a competitive salary and flexible hours. Interested applicants should co= ntact Meredith Hale, phone (214)596-2219 or e-mail mh...@carisls.com Meredith Hale HT (ASCP) CM Operations Liaison Di= rector and Education Coordinator Caris Life Sciences 6655 North MacArthur Blvd, Irving Texas 75039 direct: 214-596-2219<= BR> cell: 469-648-8253 fax: 214-596-7095 mh...@carisls.com ------------------------------ Message: 11 Date: Tue,= 24 May 2011 07:14:23 -0400 From: "Dessoye, Michael J" Subject: RE: [Histonet] p63 To: "Joanne Clark" = , Message-ID: Content-Type: text/p= lain; charset="iso-8859-1" Biocare has a very good, and fairly ine= xpensive, p63 that we have had good results with: http://biocare.net/produc ts/antibodies/p/163/ ________________________________ From: J= oanne Clark [mailto:jcl...@pcnm.com] Sent: Mon 5/23/2011 12:31 PM To:= histonet@lists.utsouthwestern.edu Subject: [Histonet] p63 I need to find a source for p63 antibody. Where does everyone purchase th= eirs from? Joanne Clark,HT Pathology Consultants of New Mexico _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ = _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are c= onfidential and intended solely for the use of the individual or entity = to whom they are addressed. If you have received this email in error = please notify the originator of the message. This footer also confirms t= hat this email message has been scanned for the presence of computer vir uses. Any views expressed in this message are those of the individua= l sender, except where the sender specifies and with authority, state= s them to be the views of Wyoming Valley Health Care System. Scannin= g of this message and addition of this footer is performed by Websense E= mail Security software in conjunction with virus detection software. ------------------------------ Message: 12 Date: Tue= , 24 May 2011 12:14:02 +0100 From: "Margaret Blount" Subject: [Histonet] Thank you To: Message-ID: Content-Type: text/plain; charset="US-ASCII" Hello everyone! Thank you all for your good wishes on my r= etirement. I knew you were all great people: you have just proved it big= time! Thank you all from the bottom of my heart. Keep smiling! Much love Margaret ------------------------------ Message: 13 Date: Tue, 24 = May 2011 13:03:21 +0000 From: abri...@brightinstruments.com Subject: = Re: [Histonet] Re: Freezing bath solutions To: mwh...@mcleodhealth.org,< BR>histonet-boun...@lists.utsouthwestern.edu, "Robert Richmond" Cc: Histonet Messa= ge-ID: <757762693-1306242203-cardhu_decombobulator_blackberry.rim.net -1362851250-@b15.c2.bise7.blackberry> Content-Type: text/plain; c= harset="Windows-1252" Melanie, In our Clini-RF rapid freezer we= have a device for keeping tissue submerged in Novec 7000. Regards <= BR> Alan Bright www.brightinstruments.com Sent from my BlackBerry= ® wireless device -----Original Message----- From: mwhite@mc= leodhealth.org Sender: histonet-boun...@lists.utsouthwestern.edu Date= : Mon, 23 May 2011 14:46:29 To: Robert RichmondCc: ; Subject: Re: [Histonet] Re: Freezing bath solutions A= llison and Dr. Richmond: We are using Novec 7000 successfully in our= freezing bath and have been doing so for about 6 months with no major p= roblems. It does have some annoying traits ; however the safety bene= fits outweigh those issues , in my opinion. The issues are: a little mor= e time required for specimen to freeze, tissues tending to float in the = solution, and evaporation. I worried that the evaporation would be a big= problem , but it has not .My techs discovered through experimentation t= hat by lowering the tissue slowly into the fluid, it would stop the flot= ation problem. Overall, I would recommend it. 3M has been very helpf= ul to us. (I'm not being paid in any way to endorse this!!) See below fo= r 3M contact information. Additionally, through earlier Histonet = inquiries, I was told about a freezing bath for those of you who still u= se the old Histobath that is no longer made. If anyone wants information= about it, It's on www.ftssystems.com and the item is called a "Mult-Coo= l Bath". We don't have one , but it looks like it could be a suitable re= placement. Here's our purchasing contact person at 3M: Christina = Cerecedes 1-888-509-5224 Melanie S. White, MT(ASCP) Labora= tory Supervisor, Systems/Anatomic Pathology McLeod Regional Medical Cent= er Florence, SC (843) 777-2072 NOTICE: This e-mail mes= sage and all attachments transmitted with it may contain legally PRIVIL= EGED and CONFIDENTIAL information intended solely for the use of the ad= dressee. If the reader of this message is not the intended recipient, y= ou are hereby notified that any reading, dissemination, distribution, c= opying, or other use of this message or its attachments is strictly pro= hibited. If you have received this message in error, please notify the = sender immediately and/or notify the postmaster (postmaster@mcleodhealt h.org), and delete this message and all copies and backups thereof. Thank Y= ou. _______________________________________________ Histonet = mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwe= stern.edu/mailman/listinfo/histonet -- BEGIN-ANTISPAM-VOTING= -LINKS ------------------------------------------------------ Tea= ch SpamSniper if this mail (ID 01EL6KSKd) is spam: Spam: http://admin.sp amsniper.co.uk/canit/b.php?i=01EL6KSKd&m=ce12d22f644f&t=20110 = 523&c=s Not spam: http://admin.spamsniper.co.uk/canit/b.php?i=01 EL6KSKd&m=ce12d22f644f&t=20110523&c=n Forget vote: htt p://admin.spamsniper.co.uk/canit/b.php?i=01EL6KSKd&m=ce12d22f64 4f&a= mp;t=20110523&c=f ----------------------------------------------= -------- END-ANTISPAM-VOTING-LINKS --------------------------= ---- Message: 14 Date: Tue, 24 May 2011 10:56:52 -0400 From: "= Cynthia Pyse" Subject: [Histonet] decal To: "'H= istonet'" Message-ID: <001101cc1a= 22$d100e9d0$7302bd70$@com> Content-Type: text/plain; charset="us-as= cii" Hello Histonetters What is everyone using to decal speci= mens. I haven't had to use it in a few years, before that I just purchas= ed formic acid and made a 10% solution. Just wanted to see if the criter= ia has changes over the years. Thanks in advance for the responses. <= BR> Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Superviso= r X-Cell Laboratories e-mail cp...@x-celllab.com <= BR> ------------------------------ Message: 15 Dat= e: Tue, 24 May 2011 08:50:13 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] decal To: Cynthia Pyse , 'Histonet' Message-ID: &= lt;259132.9447...@web65711.mail.ac4.yahoo.com> Content-Type: text/pla= in; charset=iso-8859-1 For tissues containing bone, or bone specim= ens I always used the commercial brand RDO. For bone marrow biopsi= es I used EDTA (prepared in the lab). René J. From: Cynthia = Pyse To: 'Histonet' Sent: Tuesday, May 24, 2011 10:56 AM Subject: [Histonet] decal<= BR> Hello Histonetters What is everyone using to decal specimens.= I haven't had to use it in a few years, before that I just purchased fo= rmic acid and made a 10% solution. Just wanted to see if the criteria ha= s changes over the years. Thanks in advance for the responses. Cindy Pyse, CLT, HT (ASCP) Laboratory/Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com _______________________________________________ Histonet mailing l= ist Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu= /mailman/listinfo/histonet ------------------------------ Mes= sage: 16 Date: Tue, 24 May 2011 09:01:03 -0700 (PDT) From: kristen ar= vidson Subject: [Histonet] Processor power s= upply Back-up To: histonet Messag= e-ID: <2613.13896...@web65711.mail.ac4.yahoo.com> Content-Type: te= xt/plain; charset=iso-8859-1 Does anyone have any suggestions on p= ower supply back-up for Leica ASP 300? We are an independant lab and = are not hooked up to back-up if we have an outage. Thought it might b= e a good idea :) Thanks. ------------------------------ Message: 17 Date: Tue, 24 May 2011 09:34:55 -0700 (PDT) From:= Chakib Boussahmain Subject: [Histonet] Coronavirus= antibody To: histonet@lists.utsouthwestern.edu Message-ID: <42506= 8.63659...@web161818.mail.bf1.yahoo.com> Content-Type: text/plain; ch= arset=us-ascii Hi Histonetters, We are interesting in doing imm= unostain on ferret tissues that have been fixed in formalin and embedded in= paraffin. I am just wondering if anyone used this antibody in their lab, i= f so, can you please share with us your staining protocol? antigen retrieva l protocol as well? Your help will be very much appreciated. Thank yo= u Chakib HTL(ASCP) Histology lab MIT ----------------------= -------- Message: 18 Date: Tue, 24 May 2011 11:46:17 -0500 Fro= m: "Vickroy, Jim" Subject: [Histonet] Pathology = Transmission Electron Micrscope To: "histonet@lists.utsouthwestern.edu"<= BR> Message-ID: <24A4826E8EF096 4d86bc5317306f58a55df5bec...@mmc-mail.ad.mhsil.com> Content-Type: tex= t/plain; charset="us-ascii" We are in the market to purchase a= new TEM. Our old Hitachi instrument is 31 years old and is getting tired. = The use for transmission em in pathology has somewhat diminished in our hos pital environment. We still however use TEM routinely on all renal biopsies= and nerve biopsies. Has anyone priced TEM scopes for pathology usage latel= y. In the past there have not been many options for a TEM that was for path= ology usage verses one for research. Can anyone steer me in the right direc= tion. Of course cost has become a major issue also so we are trying to make= the best choice for our usage. Thanks James Vickroy BS, HT(A= SCP) Surgical and Autopsy Pathology Technical Supervisor Memorial= Medical Center 217-788-4046 ________________________________ This message (including any attachments) contains confidential informat= ion intended for a specific individual and purpose, and is protected by law= . If you are not the intended recipient, you should delete this message. An= y disclosure, copying, or distribution of this message, or the taking of an= y action based on it, is strictly prohibited. ------------------= ------------ _______________________________________________ Hist= onet mailing list Histonet@lists.utsouthwestern.edu http://lists.utso= uthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol= 90, Issue 29 **************************************** <= /mailto:mh...@carisls.com> _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet