I am seeing this when I work with mouse tissue. After necropsy I extract the tissue from the mouse, cassette it, put it in fresh 10% NBF and let the tissue fix 24hrs before processing. I also see an edge effect when I fix with 4% PFA. During IHC-P I usually retrieve with a pressure cooker (120C) and citrate buffer solution. I use the DAKO envision kit and incubate the primary antibody at room temp. for 1 hour.
I also see an edge affect when I take frozen tissue and thaw it in RT 10%NBF overnight followed by processing. The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
