, June 19, 2014 5:24 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Eosin Leaching
Hello all,
We're having a problem with our eosin bleeding out of the sections. I've read
that this can be caused by not dehydrating adequately after eosin, but we're
still having an issue when using
Hi Histonet,
Eosin bleaching is usually due to the alcohols that are used after the eosin,
if you are in a humid area, the alcohols can actually absorb water from the
humidity in the air. This may mean a change of how often you rotate or change
the alcohols, possibly more often than you did
: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Friday, 20 June 2014 10:11 PM
To: Sanders, Jeanine (CDC/OID/NCEZID); 'Adrienne Anderson';
'histo...@listsutsouthwestern.edu'
Subject: RE: [Histonet] Eosin Leaching
Is your
...@lists.utsouthwestern.edu] On Behalf Of Sanders,
Jeanine (CDC/OID/NCEZID)
Sent: Thursday, June 19, 2014 5:26 PM
To: 'Adrienne Anderson'; 'histonet@lists.utsouthwestern.edu'
Subject: RE: [Histonet] Eosin Leaching
Have you changed your coverslipping mountant?
-Original Message-
From: histonet-boun
@lists.utsouthwestern.edu'
Subject: RE: [Histonet] Eosin Leaching
Have you changed your coverslipping mountant?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne
Anderson
Sent: Thursday, June 19, 2014 5
-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne
Anderson
Sent: Friday, June 20, 2014 8:15 AM
To: Rathborne, Toni
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Eosin Leaching
We haven't changed our mounting medium, but I'll
Subject: [Histonet] Eosin Leaching
Hello all,
We're having a problem with our eosin bleeding out of the sections. I've read
that this can be caused by not dehydrating adequately after eosin, but we're
still having an issue when using fresh alcohols. Does anyone have any other
ideas as to the cause
: [Histonet] Eosin Leaching
Hello all,
We're having a problem with our eosin bleeding out of the sections. I've read
that this can be caused by not dehydrating adequately after eosin, but we're
still having an issue when using fresh alcohols. Does anyone have any other
ideas as to the cause
Hello all,
We’re having a problem with our eosin bleeding out of the sections. I’ve read
that this can be caused by not dehydrating adequately after eosin, but we’re
still having an issue when using fresh alcohols. Does anyone have any other
ideas as to the cause of this problem?
Thanks in
Hi again,
Does anyone have an issue with eosin leaching out of sections a few days after
they have been coverslipped on an HE stained slide?
Thanks,
Scott Hendricksen HT(ASCP)
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Histonet@lists.utsouthwestern.edu
Hi again,
Does anyone have an issue with eosin leaching out of sections a few days
after they have been coverslipped on an HE stained slide?
Thanks,
Scott Hendricksen HT(ASCP)
___
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Histonet@lists.utsouthwestern.edu
Your xylene and/or alcohols may need changing.
Janet
From: histonet-boun...@lists.utsouthwestern.edu on behalf of Scott Hendricksen
Sent: Wed 2/3/2010 9:02 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Eosin leaching out of sections
Hi
...@lists.utsouthwestern.edu] On Behalf Of Scott
Hendricksen
Sent: 03 February 2010 04:16
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Eosin leaching out of sections
Hi again,
Does anyone have an issue with eosin leaching out of sections a few days
after they have been coverslipped on an HE stained
February 2010 04:16
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Eosin leaching out of sections
Hi again,
Does anyone have an issue with eosin leaching out of sections a few days
after they have been coverslipped on an HE stained slide?
Thanks,
Scott Hendricksen HT(ASCP
Of Scott
Hendricksen
Sent: 03 February 2010 04:16
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Eosin leaching out of sections
Hi again,
Does anyone have an issue with eosin leaching out of sections a few days
after they have been coverslipped on an HE stained slide?
Thanks,
Scott
Hi again,
Does anyone have an issue with eosin leaching out of sections a few days after
they have been coverslipped on an HE stained slide?
Thanks,
Scott Hendricksen HT(ASCP)
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
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