RE: [Histonet] Eosin Leaching

2014-06-24 Thread Michael LaFriniere
, June 19, 2014 5:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin Leaching Hello all, We're having a problem with our eosin bleeding out of the sections. I've read that this can be caused by not dehydrating adequately after eosin, but we're still having an issue when using

[Histonet] Eosin leaching

2014-06-23 Thread Debra Siena
Hi Histonet, Eosin bleaching is usually due to the alcohols that are used after the eosin, if you are in a humid area, the alcohols can actually absorb water from the humidity in the air. This may mean a change of how often you rotate or change the alcohols, possibly more often than you did

RE: [Histonet] Eosin Leaching

2014-06-22 Thread Tony Reilly
: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Friday, 20 June 2014 10:11 PM To: Sanders, Jeanine (CDC/OID/NCEZID); 'Adrienne Anderson'; 'histo...@listsutsouthwestern.edu' Subject: RE: [Histonet] Eosin Leaching Is your

RE: [Histonet] Eosin Leaching

2014-06-20 Thread Rathborne, Toni
...@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Thursday, June 19, 2014 5:26 PM To: 'Adrienne Anderson'; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Eosin Leaching Have you changed your coverslipping mountant? -Original Message- From: histonet-boun

Re: [Histonet] Eosin Leaching

2014-06-20 Thread Adrienne Anderson
@lists.utsouthwestern.edu' Subject: RE: [Histonet] Eosin Leaching Have you changed your coverslipping mountant? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson Sent: Thursday, June 19, 2014 5

RE: [Histonet] Eosin Leaching

2014-06-20 Thread Anita Buchiane
-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne Anderson Sent: Friday, June 20, 2014 8:15 AM To: Rathborne, Toni Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Eosin Leaching We haven't changed our mounting medium, but I'll

RE: [Histonet] Eosin Leaching

2014-06-20 Thread Vanessa Avalos
Subject: [Histonet] Eosin Leaching Hello all, We're having a problem with our eosin bleeding out of the sections. I've read that this can be caused by not dehydrating adequately after eosin, but we're still having an issue when using fresh alcohols. Does anyone have any other ideas as to the cause

RE: [Histonet] Eosin Leaching

2014-06-19 Thread Sanders, Jeanine (CDC/OID/NCEZID)
: [Histonet] Eosin Leaching Hello all, We're having a problem with our eosin bleeding out of the sections. I've read that this can be caused by not dehydrating adequately after eosin, but we're still having an issue when using fresh alcohols. Does anyone have any other ideas as to the cause

[Histonet] Eosin Leaching

2014-06-19 Thread Adrienne Anderson
Hello all, We’re having a problem with our eosin bleeding out of the sections. I’ve read that this can be caused by not dehydrating adequately after eosin, but we’re still having an issue when using fresh alcohols. Does anyone have any other ideas as to the cause of this problem? Thanks in

[Histonet] Eosin leaching out of sections

2010-02-03 Thread Scott Hendricksen
Hi again, Does anyone have an issue with eosin leaching out of sections a few days after they have been coverslipped on an HE stained slide? Thanks, Scott Hendricksen HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Eosin leaching out of sections

2010-02-03 Thread Scott Hendricksen
Hi again, Does anyone have an issue with eosin leaching out of sections a few days after they have been coverslipped on an HE stained slide? Thanks, Scott Hendricksen HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

RE: [Histonet] Eosin leaching out of sections

2010-02-03 Thread Bonner, Janet
Your xylene and/or alcohols may need changing. Janet From: histonet-boun...@lists.utsouthwestern.edu on behalf of Scott Hendricksen Sent: Wed 2/3/2010 9:02 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin leaching out of sections Hi

FW: [Histonet] Eosin leaching out of sections

2010-02-03 Thread Ian Montgomery
...@lists.utsouthwestern.edu] On Behalf Of Scott Hendricksen Sent: 03 February 2010 04:16 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin leaching out of sections Hi again, Does anyone have an issue with eosin leaching out of sections a few days after they have been coverslipped on an HE stained

RE: [Histonet] Eosin leaching out of sections

2010-02-03 Thread Joyce Cline
February 2010 04:16 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin leaching out of sections Hi again, Does anyone have an issue with eosin leaching out of sections a few days after they have been coverslipped on an HE stained slide? Thanks, Scott Hendricksen HT(ASCP

RE: [Histonet] Eosin leaching out of sections

2010-02-03 Thread Rathborne, Toni
Of Scott Hendricksen Sent: 03 February 2010 04:16 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin leaching out of sections Hi again, Does anyone have an issue with eosin leaching out of sections a few days after they have been coverslipped on an HE stained slide? Thanks, Scott

[Histonet] Eosin leaching out of sections

2010-02-02 Thread Scott Hendricksen
Hi again, Does anyone have an issue with eosin leaching out of sections a few days after they have been coverslipped on an HE stained slide? Thanks, Scott Hendricksen HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu