Hi everyone,
I have not had any success optimising the eosin counterstain in the absence of
xylene. We routinely use 0.5% eosin Y in 95% ethanol. I've tried taking
sections through to this to absolute ethanol but then air drying , but it
leaves blotches of eosin. Using eosin 0.5% aqueous with
We don't have problems with our eosin after Xylene-free deparaffining. I
use a 95% alcohol step just before the Eosin. Perhaps that might help.
We don't air dry before coverslipping as my prof finds the quality not
as good, so we use 100%IsOH before coverslipping; the mounting media you
use will
...@murdoch.edu.au
To: histonet@lists.utsouthwestern.edu
Sent: Wednesday, March 27, 2013 10:06 AM
Subject: [Histonet] Eosin not working for xylene free HE
Hi everyone,
I have not had any success optimising the eosin counterstain in the absence of
xylene. We routinely use 0.5% eosin Y in 95% ethanol
