Jim Vickroy, Histology Manager, Springfield Clinic, Springfield, Illinois asks:
>>Someone told me that you could not use eosin on the new Peloris II. Many of us use eosin to color small biopsies during the process run. We have it in one of the 95% ETOHs. Can someone explain why this can't be used in the Peoris?<< You shouldn't be coloring small biopsy specimens with eosin on any processor, because eosin's brilliant fluorescence interferes with FISH procedures (such as HER2 on breast cancers). If you want to use a red marking dye, use safranin O. The solution the microbiologists use in Gram stains works quite well, and is probably just across the hallway for you. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet