Hi Sarah, The eosin is washed out when you deparaffinize and re-hydrate the slides. If you are doing anything with the tissue without rehydrating, it could cause an autofluorescence problem, but as long as you rehydrate well, you should be fine. Incidentally, since you also asked about Golden Bridge International, they seem like a nice company. They offer free samples of their products, as long as you are willing to pay shipping. I tried their Goat polymer like this. It is worth noting however that there are a lot of anti mouse polymers that will work, so you should have quite a few choices available to you. If you need some suggestions drop me an email off list.
Have a nice weekend, Amos Message: 7 Date: Fri, 21 May 2010 14:06:40 -0700 From: sgoe...@xbiotech.com Subject: [Histonet] Eosin in Processor and iHC To: histonet@lists.utsouthwestern.edu Message-ID: < 20100521140640.9e2d9aa830e8449a2412eb1e4f2f067e.cba83845a9....@email04.secureserver.net > Content-Type: text/plain; charset="utf-8" Has anyone had any trouble prestaining tissues in eosin and then doing IHC? I'm making cell blocks from clear fluid full of cells embe be able to s in the cells be else saw an issu =) < Sarah Goebel, B.A., HT (ASCP) Histotechnician XBiotech 8201 East Riverside Dr. Bl Austin, Texas 7 (512)386-5107 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet