Hi, how the FISH signals in FFPE tissue can be strenghten? by standard procedure only weak signals for EGFR can be obtained, so far I tried different time in Vysis protease 1 (45, 60, 70 min), for other probes 70 min works quite well on our FFPE, but not for EGFR? what other modifications can I try? many thanks Irena _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
