RE: [Histonet] FITC on Ventana Ultra

2010-08-27 Thread Morken, Tim
@lists.utsouthwestern.edu Subject: [Histonet] FITC on Ventana Ultra Any users out there that have had A CAP inspector question negative control on the instrument? In regard to CAP question ANP.21850 in which the notes state, A negative reagent control in which the patient tissue is processed in an identical

Re: [Histonet] FITC on Ventana Ultra

2010-08-27 Thread Mark Tarango
Hi Nita, I agree that buffer should be used as the negative control reagent. If you have a seperate slide that is cut and put into buffer, just coverslip it with the same mounting media and you have a negative control. All the instrument is doing is putting on the antibody and then rinsing it