Is anyone out there able to help this student? We don't perform, less analyze 
these stains.
Thank you very much.
Lin.

+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
NaDH and SDH stains
We are planning to perform magnetic resonance spectroscopy on the cranial 
sartorius and vastus lateralis muscles of control dogs and dogs with Golden 
Retriever Muscular Dystrophy to evaluate for differences in the concentration 
of various metabolites and then compare this with NaDH and SDH stains of biopsy 
samples from the same sites. However, I am having some difficulty determining 
how to compare them because I do not know how these stains are normally 
analyzed; do you employ a grading scale based on the severity of change?

I am trying to confirm which statistical analyses to apply to compare the 
spectroscopic findings with the histological staining characteristics and thus 
really need to figure out exactly how the stains will be interpreted! I have 
been reading the literature to see how NaDH / SDH staining characteristics 
correlate with muscular dystrophy but it is proving a struggle. I was hoping 
you might be able to recommend a reference text?

Thank you so much in advance. If there is someone else you would prefer I 
contact about this then please do let me know.

Thanks again,

Sarah


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