Re: [Histonet] Factor XIIIa background staining problems

2012-01-31 Thread Leslie Apa
Hi Jason, I have been working with Janna on this issue and just got her email that The problem persists even after my suggestions. I can assure you the expiry is fine, many time the antibodies are still stable and usable even after the expiry date. Have you tried a variety of controls from dif

RE: [Histonet] Factor XIIIa background staining problems

2012-01-31 Thread Elizabeth Chlipala
@lists.utsouthwestern.edu Cc: Janna Hope Subject: [Histonet] Factor XIIIa background staining problems We have been experiencing Factor XIIIa background staining problems and cannot seem to find an answer to fix it. We use Cell Marque's Factor XIIIa clone EP3372 at a dilution of 1:250. We have tried

[Histonet] Factor XIIIa background staining problems

2012-01-31 Thread Jason McGough
We have been experiencing Factor XIIIa background staining problems and cannot seem to find an answer to fix it. We use Cell Marque's Factor XIIIa clone EP3372 at a dilution of 1:250. We have tried many different protocols (i.e. enzyme pretreatment, high and low pH pretreatment, different incubatio