This is quite a puzzle. I ran a handful of slides in the SAME manual run for my Masson trichrome stain (one glass caddy of slides, passing through single troughs of dyes). I've just coverslipped them and half the slides have the expected bright high contrast coloring that I usually see, and half the slides are pale/faded for all three colors.
The slides themselves came from different experiments (same wax, same thickness, but cut and de-waxed at different times) ... could it be that the paler set was not fully de-waxed? Has anyone else seen this? I would appreciate any help you can provide. Thanks! Katherine Davoli, BA, HTL(ASCP)CM Supervisor & Lab Manager, Tissue Culture & Histology Core Module Ophthalmic and Visual Sciences Research Center Department of Ophthalmology, University of Pittsburgh Mail Stop Code: EEI010901 930 Eye & Ear Institute, 203 Lothrop Street Pittsburgh, PA 15213 (412) 647-8256 davol...@upmc.edu and kdav...@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet