Dear Peter:
Our muscle freeze protocol is very easy. We employed a small piece of cork
of about 1x1cm, we pour a drop of OCT embedding medium and we placed the
muscle on it. It´s important not to pour a big drop of OCT, cause it
creates holes in the muscle as it infiltrates. Then we cooled isopent
Dear all,
Happy New Year.
We are planning to work on rat tendon and frozen section of tendon will be
performed. I'd like to ask for a better protocol to preserve, process and
section tendon as we found there is a lot of ice crystal (holes) on
sections of muscle that seriously affect our subseq
