RE: [Histonet] Frozen sectioning human spleen

2011-06-09 Thread gayle callis
HTL/HT/MT(ASCP) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of James S. Sent: Thursday, June 09, 2011 5:23 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Frozen sectioning human spleen

[Histonet] Frozen sectioning human spleen

2011-06-09 Thread James S.
I've been trying to get some frozen sections of human spleen. Usually all the work I do is with mouse tissue and I take the tissues myself and immediately freeze in OCT in isopentane/dry ice. The problem with the human samples is that I have no control over how they are frozen! I have one sample