I have always "fixed" in RT acetone for 10 mins
Have compared 0-60 mins/4C to RT acetone
Lower temps just limit the rate of reaction, imho.
I note "nuclear streaming" when I use acetone at any temp/time.
Imho, acetone is not an effective fixativeit's a delipidiser.
So, give it 10 mins at RT.
Cold acetone, and cold ethanol, were used to fix tissues because they
left enzymes unaffected and still demonstrable. This was in the early
days of enzyme histochemistry. Pearse' Histochemistry: Theoretical and
applied,3rd edition, volume 1, page 85 discusses it. I could send a scan
if you
Can anyone give me a rational for using cold (refrig or freezer-temp) acetone
to fix frozen sections? Or a rational for using RT acetone.
This is for kidney or muscle bx frozens for immmunofluroescence or
immunoperoxidase staining.
Normally they air dry for at least 15 minutes (just waiting
