Ally - I have worked with several pathologists who were color-blind -one to greens; one to blues. As a result we counterstained the GMS with different colors. A counterstain is just that. It gives a color to secondary tissue elements. Light greens, hematoxylin, metanil yellow and nuclear fast red were used. All worked well, photographed well and were the choice of the pathologist. Many older pathologists "grew up" with the procedure outlined in the AFIP manual which stated green. But other pathologists are more amiable to trying other colors. Maybe you should just as them what they prefer. Cheryl _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet