[Histonet] GMS stain on blood smear

2012-07-25 Thread Fran Pearsall
I have an aspirate from a mass on an animal. It was very bloody, I have spun down a portion of the sample. Does it need to be fixed with alcohol before I make slide smears? I need to do a GMS on a direct smear. What are the staining steps I need to do for these smears? Any info would be

[Histonet] GMS stain

2010-03-31 Thread Perry, Margaret
We sometimes have problems with the stain if we use positive slides. Margaret ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] GMS stain

2010-03-31 Thread Malika Benatti
Hi Margaret, What problem are you experiencing ? Are you over impregnated or under impregnated ? At what thickness do you cut your Section ? Ideally 3 to 4 microns How do you make up you Hexamine solution ? if you get contamination of Hexamine solution make sure that glass ware is clean. Try