[Histonet] Glycerol jelly with an anti-fading agent?

2009-03-26 Thread Pumla Pamla-Gutter
Does anyone know if it is OK to add an anti-fading agent such n-propyl gallate to glycerol jelly? I know it is ok to add it to buffered glycerol, but the problem with just using the buffered glycerol, is that it does not solidy and so my tissue sections(250umthick sections) are at risk of drying ou

RE: [Histonet] Glycerol jelly

2008-09-23 Thread Jo Dee Fish
] Glycerol jelly I've recently started using home-made glycerol jelly as my mounting medium---composition gelatin powder, water, and glycerol. I keep it in the fridge at 4 degrees and melt it before use. It's great for dark field images, but I'm worried about bacterial growth. I haven

Re: [Histonet] Glycerol jelly

2008-09-23 Thread Bryan Llewellyn
It was usually recommended to add a crystal of thymol, that should preserve it for months. Bryan Llewellyn - Original Message - From: "Pumla Pamla-Gutter" <[EMAIL PROTECTED]> To: Sent: Tuesday, September 23, 2008 11:53 AM Subject: [Histonet] Glycerol jelly I

[Histonet] Glycerol jelly

2008-09-23 Thread Pumla Pamla-Gutter
I've recently started using home-made glycerol jelly as my mounting medium---composition gelatin powder, water, and glycerol. I keep it in the fridge at 4 degrees and melt it before use. It's great for dark field images, but I'm worried about bacterial growth. I haven't had any problems yet, but I