RE: [Histonet] H&E Destain protocol

2013-02-14 Thread HERRINGTON, SHEILA
.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Maribel Santiago Sent: Thursday, February 14, 2013 11:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E Destain protocol Hello Histonet, I have samples that were stained during fixation with Eosin. First question,

[Histonet] H&E Destain protocol

2013-02-14 Thread Maribel Santiago
Hello Histonet, I have samples that were stained during fixation with Eosin. First question, since I will do IHC on them, do I need to get rid of the eosin like you do with an already H&E stained slide? If so, that brings me to my second question, does anyone have a protocol that are willing t