Here's my lab's SOP for an LHE. 7uM sections. Hope this helps!
1.Deparaffinize and hydrate to absolute ethanol.
2.Luxol Fast Blue 60° C oven 1hr.
3.Cool to room temperature.
4.Hydrate to water.
5.2 - 3 dips reducer. The fresher the reducer the less number of dips
you need.
Does anyone have a protocol/SOP for doing H&E LFB staining they would be
willing to share with me?
Diane L. Satterfield, BS
Manager Brain Tumor BioRepository
Research Program Leader
Duke University Medical Center
Brain Tumor Center Biorepository and Database
_
Morning all,
I have been asked to do this technique and just wanted to know from those of
you that have done itany tricks or do you basically do the 2 protocols
back-to-back ?
Thanks,
Jeanine H. Bartlett
Centers for Disease Control and Prevention
Infectious Diseases Pathology Branch
404-63
