[Histonet] H&E on frozen sections

2017-05-02 Thread Ana Maluenda via Histonet
Hi everyone, Just wondering if anyone out there had already had issues with commercial Haematoxylin and Eosin. I started working in a lab that recently purchased Sigma Mayer's Haematoxylin (MHS-16) and 1% Alcoholic Eosin (Fronine - II016). We work with fresh frozen sections of mouse tissue. Sin

[Histonet] H&E on frozen sections

2010-01-13 Thread Jeffrey Thompson
Hello, This is a question that has probably been asked of the group many times before. I would like to know if anyone can suggest a protocol for H&E stain of paraformaldehyde perfused rat brain sections collected at 10 microns on a cryostat? And, by the way, is there a method to search his