Hello:
Does anyone have a good manual or automatic H staining protocol that will
work well on multiple Paraffin embedded tissues?
Ken
kdea...@hotmail.com
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Obviously, you're going to get a much different result using a
polychromatic stain, than by doing a standard H But if the pathologists
like it...
I'm not even sure if they could call that stain an H in their dictation.
Seems like that would open them up to liability if, God forbid, they ever
Has anyone tried using Hologic's Thin Prep solutions (Nuclear stain, rinse,
and bluing) to do their H stains?
I am trying to validate a new stainer and in the process the pathologists
want to tweak our protocol to get a better stain and thought maybe we could
try this to see about saving money in
Does anyone have a good H stain for bone sections in glycol methacrylate.
There is alginate and collagen in the samples and it is making it very
difficult to read and differentiate between the old host bone and the new bone
or to see the osteoblasts and marrow. My information is listed below.
Hi everyone,
I have a colleague that has been asked to do H staining on a frozen specimen
collected on tape. The tape has been attached to the slide using nail polish.
Can't use the CryoJane system due to the light flash. Her question is "will the
nail polish disintegrate during the immersion
Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Monday, 29 February 2016 10:16 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] H staining
Hello all,
We've been running into an issue lately with our H staining. The hematoxylin
and eosin are light on and off but the chromatin is cons
Hi Charles,
If my memory serves, Clarifier 1 and Clarifier 2 are different mostly in that
one is an alcoholic solution and the other an aqueous one. So they both behave
differently in their mechanism of action. The alcohol based reagent should
remove more hematoxylin from the cells than the
Charles,extend time on the deparaffinization station,and even
better,renew solvents at this point also.
My kind regards,
Carlos Defeo
Histotechnologist
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