Re: [Histonet] HM 500 M cryostat issue

2010-09-23 Thread Anthony Reilly
Hi Andrew I have not used that model cryostat so this may not be the answer but I have experienced this on other cryostats when the microtome has been removed for cleaning and decontamination and reinstalled with the handle in the incorrect position resulting in the counterweight being totally

[Histonet] HM 500 M cryostat

2010-09-23 Thread Michelle MacVeigh-Aloni
Hi Andrew, I worked on different cryostats and I have memories of chuck going up. The section is cold and sturdy and I would just let it follow the chuck up as it is still attached to it. Then pull down, touch it to the knife so it sits there (it gets attached at the bottom) and pick it up. It

[Histonet] HM 500 M cryostat issue

2010-09-23 Thread Andrew Burgeson
Hello all, I have recently been using an older model MICROM HM500 M cryostat and have been experiencing an annoying problem. When I cut a section I like to leave the top edge of the OCT anchored by not cutting through it all the way. This way I can pull tension on the bottom of the section with a