[Histonet] HRP-labeled primary antibodies

Hi All, Has anyone had experience doing IHC on FFPE tissues with HRP-labeled primary antibodies?  I was wondering what the best way to detect them would be.  I assume that going strait to DAB would not work, since no amplification is there.  I was thinking of using a biotinyl tyramide step to

Re: [Histonet] HRP-labeled primary antibodies

...@yahoo.com Date: Thu, 21 May 2009 05:41:44 To: Histonethistonet@lists.utsouthwestern.edu; ih...@googlegroups.com Subject: [Histonet] HRP-labeled primary antibodies Hi All, Has anyone had experience doing IHC on FFPE tissues with HRP-labeled primary antibodies?  I was wondering what the best way

Re: [Histonet] HRP-labeled primary antibodies

: Thursday, May 21, 2009 10:48:15 AM Subject: Re: [Histonet] HRP-labeled primary antibodies If there is enough of the antigen in the tissue or sample you can detect without amplification. HRP/DAB is an enzymatic reaction so it is also is amplification. I would try it the straight up way before

RE: [IHCRG] Re: [Histonet] HRP-labeled primary antibodies

: [Histonet] HRP-labeled primary antibodies I got some great ideas. Thanks everyone! Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _ From: anh2...@med.cornell.edu anh2...@med.cornell.edu To: Kim Merriam kmerriam2...@yahoo.com; Histonet histonet@lists.utsouthwestern.edu; ih