Coming from an ancient histology tech who did IHC back in the dark ages, before automation, hand staining is a piece of cake. A couple of hints: Use a "pap" pen to circle your tissue on the slide to form a barrier to keep your reagents puddled in a concentrated spot and to minimize reagent use. Use a plastic 100 slide box as an incubation chamber. Place dampened strips of paper towels in the bottom to make a moist chamber, and lay the slides flat across the filing slots. Keep the lid closed between steps. Use a reliable kit and pre-dilutes to make life easier. Best of luck.
Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 ph: 215-938-3689 fax: 215-938-3874 Care, Comfort, and Heal _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
