Hi,
I am detecting Arc (Activity regulated cytoskeletal protein) protein in rat brain slices. I perfuse transcardially the animal with 0.1M PBS and than 4% PFA with 5% sucrose. Post fixation in 30% sucrose 1% PFA. In some cases when i take out the brain after the perfusion i make small cut or incision with the skull in the motor cortex, usually at the right side of the brain. I stain coronal 40um brain slices with primary antibody (from rabbit , santa cruz) and secondary anti rabbit Alexa488. I found very strong nuclear staining in the area around the cut in the motor cortex something like 100 um from each side. Do somebody has any idea what is the source of that staining? Generally the signal of Arc is in the nucleus and dendrites in other brain regions. I have very consistent results with Arc expression in the brain regions i am working on. The activation around the cutting area makes me worry a little. Thank you very much. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet