Hi all,
We have suddenly started losing tissue sections from our Superfrost Plus
Slides.
Our students have been cutting fixed, frozen, cryosections (20um) and
thaw-mounting these onto Superfrost Plus slides. We have suddenly
started losing lots of sections from these slides (again!!). The
Angelina,
I have seen this post a few times before and sometimes the problem was the
drying technique or making sure your water bath is clean and free of any
debris. I also remember hearing about someone pre-treating their slides with
a Trilogy (EDTA) buffer in a pressure cooker.
Good Luck!
Prepare a 1% aq. sol. of Elmer's Glue and coat the slides with it. Oven dry
and use the slides as usual.
René J.
--- On Wed, 12/9/09, Angelina Fong f...@zoology.ubc.ca wrote:
From: Angelina Fong f...@zoology.ubc.ca
Subject: [Histonet] Help! Losing sections from Superfrost Plus Slides
I would suggest you check the lot#. You may have received a bad lot and need to
get them replaced by the vendor.
Hi all,
We have suddenly started losing tissue sections from our Superfrost Plus
Slides.
Our students have been cutting fixed, frozen, cryosections (20um) and
