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From: Bob Richmond rsrichm...@gmail.com
Sender: histonet-boun...@lists.utsouthwestern.edu
Date: Mon, 9 Apr 2012 09:16:23
To: Histonet@lists.utsouthwestern.eduhistonet@lists.utsouthwestern.edu
Subject: [Histonet] HistoBath, HistoChill, Clini-RF
Terri Bishop at SPScientific sent me an e-mail about
; histonet-boun...@lists.utsouthwestern.edu; Histonet
Subject: Re: [Histonet] HistoBath, HistoChill, Clini-RF
Dear Bob,
I would just like to point out that the recommended freezing fluid for the
Bright Clini-RF Rapid -80c tissue freezer is 3M's Novec HFE-7100 not 7000 as
you state.
Best
@lists.utsouthwestern.edu
Subject: RE: [Histonet] HistoBath, HistoChill, Clini-RF
Can you use the 3M freezing fluid in a histobath instead of isopentane?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf
, Marilyn A Weiss marilyn.a.we...@kp.org,
histonet@lists.utsouthwestern.edu
Sent: Monday, April 9, 2012 7:45:38 AM
Subject: RE: [Histonet] Histobath
Fisher owns Shandon. Part of Thermo-fisher.
Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
ECOGPCO-RL
Robert. H. Lurie
Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Sunday, April 08, 2012 2:16 PM
To: 'Sherwood, Margaret'; marilyn.a.we...@kp.org;
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Histobath
Is Shandon
Terri Bishop at SPScientific sent me an e-mail about HistoChill, a
frozen section freezing bath that replaces the discontinued HistoBath.
Terri didn't feel it was appropriate for a vendor to post this
directly on HistoNet, so I am. You can contact Terri Bishop at
terri.bis...@spscientific.com
...@lists.utsouthwestern.edu] On Behalf Of Sherwood,
Margaret
Sent: Friday, April 06, 2012 12:52 PM
To: 'marilyn.a.we...@kp.org'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Histobath
I googled Histobath and Shandon sells them, plus some other on-line
companies. Check it out.
Peggy
: Tue, 12 Jul 2011 19:16:59 -0500
From: techon...@comcast.net
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Histobath revised
Hey again.
I was not refering to a tissue flotation bath. The Histobath was a
product sold by Shandon. It had a chamber filled with isopentane
Hey All,
I am working with a company to develop a replacement for the Histobath.
They should have a prototype to me by the end of the week. I am looking
for a lab, preferably in the Chicago, that is willing to test and assess
the product. Let me know if you are interested.
Thanks
Matt
--
Hey again.
I was not refering to a tissue flotation bath. The Histobath was a
product sold by Shandon. It had a chamber filled with isopentane or
alcohol and was used for quick freezing tissue for a cryostat. Sorry if
there was any confusion.
Thanks
Matt
--
Matthew Mincer
Tech One
Does anyone know the normal operating temperature of the Thermo
Histobath? A customer has asked me to repair theirs and I want to make
sure I get the temp correct.
Thanks
Matt
--
Matthew Mincer
Tech One Biomedical Service
159 N Marion Street
PMB163
Oak Park, IL 60301
office (708) 383-6040 X
Hi Matt,
Depending on the wax melting point which should be around 57 degree Celsius,
your water bath should be set at 50 degree Celsius.
Hope this help.
Malika Benatti BSc MIBMS
Specialist Biomedical Scientist
Great Ormond Street Children Hospital
London, WC1N 3JH
UK
Tel: (+44) 0207 4059200
I forgot to mention, this Histobath I am talking about is the one with a
refrigerated tank. You fill the tank with a liquid (I forget what the
chemical is) and use it to quick-freeze tissue to be cut in a cryostat.
Peace
Matt
--
Matthew Mincer
Tech One Biomedical Service
159 N Marion Street
Matt,
I have a copy of the instructional/operational manual from my histobath
that we purchased in 1994 from Shandon-Lipshaw. The specifications for
its lowest temperature is -60C. The liquid that is used is
2-methylbutane (isopentane).
I can fax you a copy if it would help.
Lynette
Lynette
Sorry for that Matt,
In this case the internal temperature of the cryostat chamber should be set
at -20 to -22 degree Celsius, not sure what would the actual temperature of
the histobath itself but our thermo scientific cryostat has the following
settings:
- the cryobar is set at -18 Degree
Hello,
I am looking for a replacement for my Shandon Histo-bath. It is a
snap freeze instrument that I have been using with Isopentane.
Do you have something comparable to this or any suggestion? My
Histobath finally gave up.
Many thanks,
Deborah K. Edwards, HTL/QIHC, (ASCP)
edwards.debor...@va.gov
Date: Wed, 30 Sep 2009 15:04:00
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Histobath Frozen Sections
Hello,
I am looking for a replacement for my Shandon Histo-bath. It is a
snap freeze instrument that I have been using with Isopentane.
Do you have something
I have noted postings by Bob Richmond and others concerning nonflammable
alternatives to hydrocarbon bath fluids for Histobaths. I work as a heat
transfer Application Engineer in the division of 3M that manufactures the
Novec line of fluids including the HFE-7100 that has been mentioned. I do
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