Thanks for citing the article I helped participate in!
Sent from my iPhone
> On Jul 7, 2015, at 2:25 PM, Terri Braud wrote:
>
>
> In the article reference below, they estimate tissue loss at 7%. In my lab,
> our pathologists would croak if we had tissue loss at this high of a rate.
> But
In the article reference below, they estimate tissue loss at 7%. In my lab,
our pathologists would croak if we had tissue loss at this high of a rate. But
with that said, we do have a 0.8% "loss of tissue". Without exception, they
have been extremely small pieces that could have been mucous,
Steven,
The problems associated with tissues lost are quite similar to those with
floaters.
Here is a newly edited copy of what I posted a few years ago on floaters and
you may find it useful.
We still wrap nearly all specimens. At times we cannot see minute flakes of
tissue, but retrieve them
Alkaline Phosphatse bubbles
Hi Tim,
Use Dako ultramount. The bubbles are caused by the naphthyl breaking down
and releasing CO2 under the coverslip. Apply small amount of the ultramount
to cover the muscle tissue and bake in 65 degree oven of 15 to 20 minutes.
If you bake it too long the myofibers
