I wonder if streptavidin Alexa (495 or 488) can be use to bind to biotinlyted primary. So no worry about species issue? May need to do biotin blocking first to get ride of endogenic biotin I guess.
Dorothy Hu Sent from my iPad > On Feb 20, 2016, at 1:00 PM, histonet-requ...@lists.utsouthwestern.edu wrote: > > Double stain IHC question _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet