I agree w Dr. Richmond. > > Message: 2 > Date: Sun, 31 Jul 2016 17:43:20 -0400 > From: Bob Richmond <rsrichm...@gmail.com> > To: "Histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Subject: Re: [Histonet] B-5 Fixative > Message-ID: > <caoksrh6g8rousicfdp5gkpeayjefb+gyw1ixgxsc14138gs...@mail.gmail.com> > Content-Type: text/plain; charset=UTF-8 > > Jillian A. Russell, HT (ASCP)CM, QIHCCM, Supervisor, CDx Histology > Operations, R&D at Dako in Carpinteria CA asks: > >>> I am wondering how many labs are using B-5 fixatives and how many are > using B-5 alternatives due to the mercury issue? - For those who have > switched to an alternative, have you noticed many differences from the > traditional B-5 fixative?<< > > This is a very controversial topic. B-5 fixative contains a large quantity > of mercuric chloride, along with formaldehyde, and you really can't use it > any more. I gave up B-5 and Zenker's fixatives very reluctantly. > > My personal opinion is that B-5 substitutes are no improvement on neutral > buffered formalin, and may compromise immunostains (which often specify NBF > fixation). > > Bob Richmond >
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