Hello all! If any licensed Histotechs are interested in working in the San Francisco Bay area, Kaiser Permanente in Berkeley is hiring! Please send me an email so I can assist on getting you on board!
On Sep 22, 2016 10:17 AM, <histonet-requ...@lists.utsouthwestern.edu> wrote: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Positive Control for TRAP Staining (Jessica Riggleman) 2. CAP Inspection (Adesupo, Adesuyi (Banjo)) 3. Unprocessing tissue from paraffin (Caroline Miller) 4. Mousse tissue processing (Blanca Lopez) 5. Quotes for capital (Record, Angela N) 6. Re: Mousse tissue processing (Caroline Miller) 7. Re: Unprocessing tissue from paraffin (Maria Mejia) ---------------------------------------------------------------------- Message: 1 Date: Wed, 21 Sep 2016 17:05:30 +0000 From: Jessica Riggleman <jriggle...@globusmedical.com> To: "'histonet@lists.utsouthwestern.edu' (histonet@lists.utsouthwestern.edu)" <histonet@lists.utsouthwestern.edu> Subject: [Histonet] Positive Control for TRAP Staining Message-ID: <D0AEE53F1F084340888BEB10C81DE9B295C5BB88@MBX2.gminc.local> Content-Type: text/plain; charset="utf-8" Hello, Does anyone have a suggestion for a good positive control for TRAP staining? Thank you, Jessica Riggleman _____________________________________________________________________ Jessica Riggleman | Research Associate Globus Medical, Inc. Valley Forge Business Center 2560 General Armistead Avenue | Audubon, PA 19403 Ph: (610) 930-1800 ext. 2583 | Fax: Confidentiality Note: This email is confidential and intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing, or copying of this email is strictly prohibited. If you have received this email in error please contact the sender. Any views or opinions presented are solely those of the author and do not necessarily represent those of Globus Medical, Inc. Although this email and any attachments are believed to be free of any virus or other defects which might affect any computer or IT system into which they are received, no responsibility is accepted by Globus Medical, Inc. for any loss or damage arising in any way from the receipt or use thereof. ------------------------------ Message: 2 Date: Wed, 21 Sep 2016 12:54:06 -0500 From: "Adesupo, Adesuyi (Banjo)" <abades...@nrh-ok.com> To: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Subject: [Histonet] CAP Inspection Message-ID: <04ee4f75bb5fb246adb68d69b7460443b1006b6...@mail.nrhnt.nrh-ok.com> Content-Type: text/plain; charset="us-ascii" Hi, We had our CAP Inspection last week. I had two Pathologists and a Cytotechnologist (we don't do cytology) with me all day. There was no Histotechnologist on the team. I am just curious, whether any of you have had this type of inspection before. Best regards, Banjo Adesuyi ====================================== CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. ------------------------------ Message: 3 Date: Wed, 21 Sep 2016 12:38:00 -0700 From: Caroline Miller <mi...@3scan.com> To: "Histonet@Lists. Edu" <histonet@lists.utsouthwestern.edu> Subject: [Histonet] Unprocessing tissue from paraffin Message-ID: <cafthrqpasucgtfx-eo2dfyrjg_gbthn79pox9a-douz50es...@mail.gmail.com> Content-Type: text/plain; charset=UTF-8 Hi there Histonetters! I am currently working through some issues of unprocessing tissue to then restain 'whole mount' with various stains, and then reprocess back to paraffin. I am running into issues of tissue being brittle and scratchy tissue after reprocessing and sectioning. Can anyone offer any advice on kinder dewaxing procedures and / or additives to the unprocessing / processing fluids that might help in the resulting piece of tissue? I have currently been putting it through the cleaning cycle on the processor, so standard xylene then alcohol treatment. thanks in advance for your advice! mills -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 ------------------------------ Message: 4 Date: Wed, 21 Sep 2016 19:59:17 +0000 From: Blanca Lopez <blanca.lo...@utsouthwestern.edu> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: [Histonet] Mousse tissue processing Message-ID: <0d9b69779c50481c8c1be992255a8...@swms13mail12.swmed.org> Content-Type: text/plain; charset="us-ascii" Hello! Histonets. Does anyone has a good protocol for processing mouse tissue? Somebody can share. thanks Blanca Lopez Histotech (ASCP) UTSW Tissue Resource K1.210 Simmons Comprehensive Cancer Center UT Southwestern Medical Center Telephone: 214-648-7598 Email: blanca.lo...@utsouthwestern.edu ________________________________ UT Southwestern Medical Center The future of medicine, today. ------------------------------ Message: 5 Date: Wed, 21 Sep 2016 20:06:07 +0000 From: "Record, Angela N" <anrec...@saintfrancis.com> To: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Subject: [Histonet] Quotes for capital Message-ID: <20FC170D97CF6546939ABB0FA907C8DB039C70@SFHSEXCH001.WarrenNT. SaintFrancis.Loc> Content-Type: text/plain; charset="us-ascii" Greg, Could you please send me 2 separate budgetary quotes for a VIP6 and a Prisma? Thank You, Angela Record, BS, HT(ASCP) Saint Francis Hospital Tulsa, OK 918-494-1321 ---------------------------------------------------------------------- Saint Francis Health System intends this email only for the use of the person to whom it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If you have received this email in error, you are hereby notified that we do not consent to any reading, dissemination, distribution, or copying of this email and request you notify the sender immediately and destroy this transmission. Violators may be prosecuted under Federal law. ------------------------------ Message: 6 Date: Wed, 21 Sep 2016 15:37:03 -0700 From: Caroline Miller <mi...@3scan.com> To: Blanca Lopez <blanca.lo...@utsouthwestern.edu> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Mousse tissue processing Message-ID: <cafthrqoq3w6b+ac7p4f5-gsyzr_90joihs64lf_l30x9kzu...@mail.gmail.com> Content-Type: text/plain; charset=UTF-8 Hi Blanca, This is what I use, note, no formalin on my machine, and you could probably cut out one of the last alcohols Step Routine tissue 70% alcohol 10 80% alcohol 10 90% alcohol 20 100% alcohol 20 100% alcohol 20 100% alcohol 30 100% alcohol 30 100% alcohol 30 Xylene 30 Xylene 35 paraffin 30 paraffin 30 paraffin 30 paraffin 30 paraffin at 60oC, P/V on, agit on On Wed, Sep 21, 2016 at 12:59 PM, Blanca Lopez via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hello! Histonets. > Does anyone has a good protocol for processing mouse tissue? Somebody can > share. > thanks > > Blanca Lopez > Histotech (ASCP) > UTSW Tissue Resource K1.210 > Simmons Comprehensive Cancer Center > UT Southwestern Medical Center > Telephone: 214-648-7598 > Email: blanca.lo...@utsouthwestern.edu > > > ________________________________ > > UT Southwestern > > > Medical Center > > > > The future of medicine, today. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 ------------------------------ Message: 7 Date: Wed, 21 Sep 2016 19:10:06 -0700 From: Maria Mejia <mbmph...@gmail.com> To: Caroline Miller <mi...@3scan.com> Cc: "Histonet@Lists. Edu" <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Unprocessing tissue from paraffin Message-ID: <a430919d-af5e-4474-84e7-541844cda...@gmail.com> Content-Type: text/plain; charset=utf-8 Hi Millis, Lets see if I?m understanding you correctly. 1) You have unprocessed tissue. What kind of unprocessed tissue & how thick are the free-floating sections? 2) Are the tissue fixed? If so, with what? & For how long? 3) You want to stain the unknown whole mount using various histochemical stains or IHC stains? 4) Can you please explain why after staining you need to reprocess for paraffin? Cause once these mystery sections are processed you'll have to cut them using a rotary microtome. I can maybe help you, but your going to have to provide more detail information. Also provide your processing protocol. Regards Maria Mejia Lead Histologist Memory & Aging Center UCSF > On Sep 21, 2016, at 12:38 PM, Caroline Miller via Histonet < histonet@lists.utsouthwestern.edu> wrote: > > Hi there Histonetters! > > I am currently working through some issues of unprocessing tissue to then > restain 'whole mount' with various stains, and then reprocess back to > paraffin. > > I am running into issues of tissue being brittle and scratchy tissue after > reprocessing and sectioning. > > Can anyone offer any advice on kinder dewaxing procedures and / or > additives to the unprocessing / processing fluids that might help in the > resulting piece of tissue? > > I have currently been putting it through the cleaning cycle on the > processor, so standard xylene then alcohol treatment. > > thanks in advance for your advice! > mills > > > > > -- > Caroline Miller (mills) > Director of Histology > 3Scan.com > 415 2187297 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 154, Issue 18 ***************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet