When you fix the slide in 10% formalin you will have to perform AR.
TH
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Sent: Wednesday, March 15, 2017 12:00 PM
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Subject: Histonet Digest, Vol 160, Issue 15
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Today's Topics:
1. Immunoperoxidase Protocol on Cytospin (Margaryan, Naira)
2. Re: Immunoperoxidase Protocol on Cytospin (Cartun, Richard)
3. Congo red and LED illumination problem? (Morken, Timothy)
4. Day Shift Histotech Job in Southern CA (Melissa Owens)
5. Bone Processing (MICHELLE BETH TITUNICK)
6. GSH Annual Histopalooza April 21st - April 23rd Legacy Lodge,
Lake Lanier Islands, Georgia (Zimmerman, Billie)
----------------------------------------------------------------------
Message: 1
Date: Tue, 14 Mar 2017 18:50:45 +0000
From: "Margaryan, Naira" <nmargar...@luriechildrens.org>
To: "histonet-boun...@lists.utsouthwestern.edu"
<histonet-boun...@lists.utsouthwestern.edu>
Cc: histonet-request <histonet@lists.utsouthwestern.edu>
Subject: [Histonet] Immunoperoxidase Protocol on Cytospin
Message-ID:
<34b2eda118548a4eb35d6e650345ba6469c06...@sv-ex08.childrensmemorial.org>
Content-Type: text/plain; charset="utf-8"
Dear histonetters:
I never done ICC.
A scientist brought me a slide with Cytospined cells on for IHC.
I am going to fix with 10%NBF (it is only what I have now) then wash with TBST.
May I skip AR step?
May I use my usual IHC protocol and reagents which I usually use for FFPE
tissue: blocks with H2O2, avidin/biotin/PB with TBST wash buffer????
Thanks in advance,
Naira
Ranked nationally in all 10 pediatric specialties by U.S. News & World Report
(LCHOC Ver 1.0)
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------------------------------
Message: 2
Date: Tue, 14 Mar 2017 19:38:08 +0000
From: "Cartun, Richard" <richard.car...@hhchealth.org>
To: "Margaryan, Naira" <nmargar...@luriechildrens.org>
Cc: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Subject: Re: [Histonet] Immunoperoxidase Protocol on Cytospin
Message-ID:
<9215bd4b0ba1b44d962a71c758b68d2e95401...@hhcexchmb03.hhcsystem.org>
Content-Type: text/plain; charset="us-ascii"
Since you only have 1 slide I would recommend following your regular protocol
that you use for formalin-fixed, paraffin-embedded tissue. I would use heat
retrieval if that's what you use for FFPE tissue. However, if you use enzyme
digestion on FFPE tissue I would "not" digest your cytospin slide. If you
don't get any immunoreactivity it could be a "False Negative" due to
pre-analytical variables. I think it can be interesting to try experiments
like this, but it's not the way to do IHC testing.
Richard
Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 972-1596
(860) 545-2204 Fax
-----Original Message-----
From: Margaryan, Naira [mailto:nmargar...@luriechildrens.org]
Sent: Tuesday, March 14, 2017 3:24 PM
To: Cartun, Richard
Subject: RE: Immunoperoxidase Protocol on Cytospin
cytoplasmic staining for Nodal protein.
thanks
________________________________________
From: Cartun, Richard [richard.car...@hhchealth.org]
Sent: Tuesday, March 14, 2017 2:13 PM
To: Margaryan, Naira; histonet-boun...@lists.utsouthwestern.edu
Subject: RE: Immunoperoxidase Protocol on Cytospin
What is the target?
Richard
Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic
Proteomics Laboratory Director, Biospecimen Collection Programs Assistant
Director, Anatomic Pathology Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 972-1596
(860) 545-2204 Fax
-----Original Message-----
From: Margaryan, Naira via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, March 14, 2017 2:51 PM
To: histonet-boun...@lists.utsouthwestern.edu
Cc: histonet-request
Subject: [Histonet] Immunoperoxidase Protocol on Cytospin
Importance: High
Dear histonetters:
I never done ICC.
A scientist brought me a slide with Cytospined cells on for IHC.
I am going to fix with 10%NBF (it is only what I have now) then wash with TBST.
May I skip AR step?
May I use my usual IHC protocol and reagents which I usually use for FFPE
tissue: blocks with H2O2, avidin/biotin/PB with TBST wash buffer????
Thanks in advance,
Naira
Ranked nationally in all 10 pediatric specialties by U.S. News & World Report
(LCHOC Ver 1.0)
This message contains confidential information and is intended only for the
individual named. If you are not the named addressee you should not
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destroyed, arrive late or incomplete, or contain viruses. The sender therefore
does not accept liability for any errors or omissions in the contents of this
message, which arise as a result of e-mail transmission. If verification is
required please request a hard-copy version. (LCHOC VER 1.0)
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------------------------------
Message: 3
Date: Tue, 14 Mar 2017 19:42:36 +0000
From: "Morken, Timothy" <timothy.mor...@ucsf.edu>
To: Histonet <histonet@lists.utsouthwestern.edu>
Subject: [Histonet] Congo red and LED illumination problem?
Message-ID:
<761e2b5697f795489c8710bcc72141ff8e5c1...@ex07.net.ucsf.edu>
Content-Type: text/plain; charset="us-ascii"
Something new to me... A pathologist said he is getting reports that LED
illumination on newer microscopes is leading to faint or even false negatives
for congo red amyloid birefringence due to the difference in spectrum between
LED and tungsten filament lamps. Has anyone else noticed this?
Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
------------------------------
Message: 4
Date: Wed, 15 Mar 2017 00:47:09 +0000
From: Melissa Owens <meli...@alliedsearchpartners.com>
To: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Subject: [Histonet] Day Shift Histotech Job in Southern CA
Message-ID: <d4ee0747.35ee7%meli...@alliedsearchpartners.com>
Content-Type: text/plain; charset="us-ascii"
Hello Histoland,
I have a Day Shift Histotech opportunity in Southern California for full time
permanent employment. Please contact me directly for more details.
Melissa Owens
President, Laboratory Staffing
Allied Search Partners
T: 888.388.7571 ext. 102
F: 888.388.7572
------------------------------
Message: 5
Date: Wed, 15 Mar 2017 09:58:08 -0400
From: "MICHELLE BETH TITUNICK" <mbt...@psu.edu>
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Bone Processing
Message-ID: <1489586288l.21430310l...@psu.edu>
Content-Type: text/plain; charset=utf-8
Hello. Does anyone have a processing protocol for formalin fixed decalcified
rat bones using an automated processor? I've been doing it manually with a
vacuum oven and it takes 14-16 hours to complete. I don't have a vacuumed
section on my automatic processor.
Also any tips besides what I've read in the archives for immunohistochemistry?
Thanks so much.
Michelle B. Titunick
PhD Candidate, Anatomy
The Pennsylvania State University College of Medicine
mbt...@psu.edu
(973) 715-9831
------------------------------
Message: 6
Date: Wed, 15 Mar 2017 15:21:13 +0000
From: "Zimmerman, Billie" <bzimm...@augusta.edu>
To: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Subject: [Histonet] GSH Annual Histopalooza April 21st - April 23rd
Legacy Lodge, Lake Lanier Islands, Georgia
Message-ID:
<mwhpr03mb2607cb3447f7389662f02637cb...@mwhpr03mb2607.namprd03.prod.outlook.com>
Content-Type: text/plain; charset="us-ascii"
The Georgia Society for Histology Histopalooza is just around the corner!!
Calling all procrastinators to register for the event.
It's cheap, cheap, cheap compared to other CEU events. I spoke with Claudia
Faulkenberry this morning.
She will be speaking about the hot, hot antibody PDL1. (no, it ain't cheap )
It's on television commercials so the general public is asking about it. She
will tell us the uses for this antibody as well as the various clones
available. Companion diagnostics is the new buzz word for PDL1. Thank you
Claudia.
See you at Lake Lanier,
Billie Zimmerman MT(ASCP)QIHC
------------------------------
Subject: Digest Footer
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End of Histonet Digest, Vol 160, Issue 15
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