Re: How to prevent bubbling in pressure cooker for immunohistochemistry procedure.
Maria Thank you so much for your feedback. The instrument i use for the antigen retrieval process is just household 6L Russek Hobbs pressure cooker. Before i place my slides into this PC i do actually wait for it to boil and actually do see the solution bubble. Then only do i put my slides in and close the lid. I am assuming it continues to bubble while reaching its optimum temperature, but you think not, so i am unsure. This procedure works quite well as we get good results. However, i do get an odd 1 or 2 tissues washing off now and again. So to try and stop this completely and to perfect our method i thought about this article i read, but sadly have lost. As i do think that maybe no bubbling will prevent this. But could be wrong. Will keep searching for the info i lost. Thanks again, keep well. Best Regards Subash Get Outlook for Android<https://aka.ms/ghei36> ________________________________ From: histonet-requ...@lists.utsouthwestern.edu <histonet-requ...@lists.utsouthwestern.edu> Sent: Thursday, October 17, 2019 7:00:01 PM To: histonet@lists.utsouthwestern.edu <histonet@lists.utsouthwestern.edu> Subject: Histonet Digest, Vol 191, Issue 10 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet<https://protect-za.mimecast.com/s/i7J9CAnX51i7ODBMtMu1Qc> or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: How to prevent bubbling in pressure cooker for immunohistochemistry procedure (Maria Cruz) 2. Re: ADH5 multiplex procedure (Sally Price) 3. Re: cleaning old charged slides (Atoska Gentry) 4. procuring fresh tumor tissue (Curt Tague) 5. Re: cleaning old charged slides (Dave Juliano) 6. ICYMI here is a link to part 3 of 3 in my series on Relocation - Relocation an Action Plan. And our latest histology opportunties on and off the bench! (Pam Barker) ---------------------------------------------------------------------- Message: 1 Date: Wed, 16 Oct 2019 13:43:28 -0400 From: Maria Cruz <mcruz8...@gmail.com> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] How to prevent bubbling in pressure cooker for immunohistochemistry procedure Message-ID: <capg7ww1f0dsbw85m8wnrz2pamxpgkqpxw0v9kx-svxmwbv1...@mail.gmail.com> Content-Type: text/plain; charset="UTF-8" Subash: Since the main purpose of using a pressure cooker for HIER is the rapid and reliable pre-treatment of sections while PREVENTING boiling and potential tissue detachment, you really shouldn?t be experiencing any bubbling. If you are, then your PC isn?t sealed adequately and the only way to fix that problem is to replace the gasket or find another way to tighten the lid.. I?ve never heard of anything that you can put inside of a PC that can change the way it pressurizes, so I?ll be following this thread to see if anyone else can contribute. Maria ************************************** Hi there all Can anybody please tell me if they have heard of or perhaps know of a substance that can be placed in the pressure cooker during the antigen retrieval process for immunohistochemistry. This prevents excessive bubbling and lifting up of sections. I did somehow come across such info a while back, but seem to have lost it. Many thanks. Best Regards Subash Govender ------------------------------ Message: 2 Date: Wed, 16 Oct 2019 15:06:02 -0400 From: Sally Price <sprice2...@gmail.com> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] ADH5 multiplex procedure Message-ID: <cahonc4dwo7tkxhngxavjfsr60og9y-oqee6whdyu8ho9yhm...@mail.gmail.com> Content-Type: text/plain; charset="UTF-8" ---------- Forwarded message ---------- From: "Kurth Virginia L." <vku...@uwhealth.org> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Virginia: I?m pretty certain that the ADH5 multiplex procedure is performed at CSI Labs in the Atlanta area. And I don?t think that biocare refers to it by that name any more either - I think they just spell out all the antigens that the antibodies in this cocktail can identify, like CK-5/14+p63+CK-7/18. Sally - - - - - - - - - - - Date: Tue, 15 Oct 2019 14:48:40 Subject: [Histonet] ADH5 Does anyone know if there is a reference lab that performs ADH5. (particular the BioCare multiplex stain?) Thanks Ginny-- Sally Price ------------------------------ Message: 3 Date: Thu, 17 Oct 2019 15:16:58 +0000 From: Atoska Gentry <gent...@auburn.edu> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] cleaning old charged slides Message-ID: <bn6pr19mb0033d04f8ec29f2953806bcec7...@bn6pr19mb0033.namprd19.prod.outlook.com> Content-Type: text/plain; charset="us-ascii" Hello, Has anyone had success cleaning old charged slides which have become foggy over time. Thanks! ~Atoska ------------------------------ Message: 4 Date: Thu, 17 Oct 2019 16:00:18 +0000 From: Curt Tague <c.ta...@pathologyarts.com> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: [Histonet] procuring fresh tumor tissue Message-ID: <byapr14mb2533884bb27db71e43c5e461f1...@byapr14mb2533.namprd14.prod.outlook.com> Content-Type: text/plain; charset="us-ascii" Hello again histonet-ers- I am working with a company do validate a new rapid (another new) tissue processor... we are currently in the process of submitting a proof of concept paper to CAP then will submit a formal white paper with data. What I am curious about is locating tumor tissue that can be used to parallel this study, obviously one part processed with the standard process we are all accustomed to and the other piece processed with this new technology. We are running all the necessary tests to show consistency and comparable results, H&E, special stains, IHC, DNA and RNA extraction... what I could use a little help with is sourcing some tumor that has not been in formalin for weeks and weeks... specifically breast tumor and possibly lung tumor to demonstrate all the downstream tests are not compromised with this technology. Is there any source out there someone could recommend and/or, if you are in a large hospital setting, is that something you might be able to assist with, when a tumor is identified and not entirely needed for diagnostics? All the best to you all, thanks for your thoughts, Curt ------------------------------ Message: 5 Date: Thu, 17 Oct 2019 09:21:09 -0700 From: Dave Juliano <dave.c.juli...@gmail.com> To: Atoska Gentry <gent...@auburn.edu> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] cleaning old charged slides Message-ID: <CAKxzVt+yD0w1Bs2QqDHBc1Jrr5d3TudY=sb9+wn2ralw5n3...@mail.gmail.com> Content-Type: text/plain; charset="UTF-8" Hi Atsoka! I?d be willing to trade you fresh slides for those foggy ones- studying effects of age on charges slides. Contact me at: Dave.c.juliano at gmail dot com if you?re interested! On Thu, Oct 17, 2019 at 8:24 AM Atoska Gentry via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hello, > > Has anyone had success cleaning old charged slides which have become foggy > over time. > > Thanks! > ~Atoska > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet<https://protect-za.mimecast.com/s/i7J9CAnX51i7ODBMtMu1Qc> > ------------------------------ Message: 6 Date: Thu, 17 Oct 2019 12:27:50 -0400 From: "Pam Barker" <rel...@earthlink.net> To: "Histopeeps Histonet" <histonet@lists.utsouthwestern.edu> Subject: [Histonet] ICYMI here is a link to part 3 of 3 in my series on Relocation - Relocation an Action Plan. And our latest histology opportunties on and off the bench! Message-ID: <014d01d58507$d24bdcb0$76e39610$@earthlink.net> Content-Type: text/plain; charset="iso-8859-1" Hi Histonetters, ICYMI - In case you missed it!! I am so excited!! My latest article has been published in the NSH Quarterly Career Center Newsletter under my byline: Make the Cut My latest article is entitled: Relocation ? An Action Plan This is part 3 of 3 in my series on relocation. You can find a link to parts 1&2 in this article as well! This is the link to my article on the NSH?s Fixation on Histology Blog and it is also in the current edition of the NSH Career Newsletter. Here is the link to the article: https://www.fixationonhistology.com/post/relocation-part-3-action-plan<https://protect-za.mimecast.com/s/Y4K7CBgX56fEKL03tvyFb_> If you have a minute to read it I would love to hear what you think. If you can?t get to the article with this link let me know and I will send you a copy of it. Histopeeps, we also have amazing opportunities nationwide! Leadership and Specialty opportunities: 1. Chicago Greater Chicago area-Field Applications Specialist ? IHC 2. Boston Greater Boston area-Field Applications Specialist ? IHC 3. Los Angeles Manager of Customer Training and Support 4. Nashville Histology Supervisor 5. Kalamazoo Applications Specialist 6. Orlando Histology Instructor HT or HTL or elig opportunities: 1. California Northern CA IHC Specialist 2. California Northern CA Histology tech 3. N. Carolina Raleigh/Durham area Histology Tech 4. Colorado Denver area ? IHC Histology Tech 5. Wisconsin Milwaukee ? Histology Tech 6. Georgia Columbus ? Histotech dermpath 7. S. Carolina Beaufort ? Histology Tech Please take a look and if you are interested let me know. If you have a friend who is interested and I place them then I get to give you a referral reward! My clients offer competitive pay rates, excellent benefits and in most cases Relocation assistance or a sign on bonus. They are ready to interview and hire!!! If You or Anyone You Know Might Be Interested In a New Opportunity, Please Contact Me ASAP call or text me on my cell At 407-353-5070. If you want some additional information or to set up a time to chat please call me toll free at 866-607-3542 or email me at rel...@earthlink.net Have a great day. I look forward to hearing back from you. Thanks-Pam #jobs4myhistopeeps #ilovemyhistopeeps #histopeeps Follow my hashtags and make your day great and your career greater!! Right Place, Right Time, Right Move with RELIA! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???? (407)353-5070 FAX:???? (407)678-2788 E-mail: rel...@earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA<https://protect-za.mimecast.com/s/qexfCDRZ58igA8wQtB-k9e> www.linkedin.com/in/reliasolutions<https://protect-za.mimecast.com/s/bnAfCElX5xiRxZOKhyixty> www.twitter.com/pamatrelia<https://protect-za.mimecast.com/s/K1wLCGZXj8tj4zEoFWfpgB> ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet<https://protect-za.mimecast.com/s/i7J9CAnX51i7ODBMtMu1Qc> ------------------------------ End of Histonet Digest, Vol 191, Issue 10 ***************************************** Disclaimer - University of Cape Town This email is subject to UCT policies and email disclaimer published on our website at http://www.uct.ac.za/main/email-disclaimer or obtainable from +27 21 650 9111. 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