STOP On Sat, Nov 13, 2021, 11:00 AM <histonet-requ...@lists.utsouthwestern.edu> wrote:
> Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-requ...@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-ow...@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > Today's Topics: > > 1. Re: Paraffin embedding following storage in 70% alcohol > (John Kiernan) > > > > ---------- Forwarded message ---------- > From: John Kiernan <jkier...@uwo.ca> > To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu>, > Charles Riley <cri...@udel.edu> > Cc: > Bcc: > Date: Fri, 12 Nov 2021 22:42:06 +0000 > Subject: Re: [Histonet] Paraffin embedding following storage in 70% alcohol > Of course you are right! > This is yet another example of an error in a procedure informally handed > on from person to person! Always work from a book. Even a very old one will > be OK for paraffin embedding. > John Kiernan. > > https://www.schulich.uwo.ca/anatomy/people/faculty/emeriti/kiernan_john.html > = = = > ________________________________ > From: Charles Riley via Histonet <histonet@lists.utsouthwestern.edu> > Sent: November 12, 2021 11:03 AM > To: histonet@lists.utsouthwestern.edu <histonet@lists.utsouthwestern.edu> > Subject: [Histonet] Paraffin embedding following storage in 70% alcohol > > I am working with a grad student on a project dealing with equine articular > cartilage. > > The protocol she sent me for embedding the tissue samples goes directly > from 70% alcohol to the embedding step in paraffin. > > > Correct me if I am wrong but shouldn't the tissue be dehydrated fully and > cleared before embedding the samples? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet