Thank you all very much for your suggestions. I'm going to play around with
a progressive H when I return from vacation next month. I do have
safranin on hand but will need to order some phloxine to experiment with. I
will probably need to order some additional supplies to make the
hematoxylin.
Angela:"Pale" results are the trade-off for great quality very thin "2 µm"
sections but you can always improve intensity somewhat .1- your "regressive"
stain, if it is "modern Harris" has the inherent problem of lacking mercury
chloride and it is little you can do about. Perhaps if you use
When I cut at 2μm my H and special stains look pale. How can I get my
stains to pop or am I stuck with pale looking stains when sectioning that
thin?
I run manual specials and a manual regressive H For H I've tried
increasing my time in hematoxylin (beyond the manufacturer recommendation),
