Re: [Histonet] How to process tissues submitted in acrylamide

2014-01-24 Thread John Kiernan
If it's a cross-linked polyacrylamide gel (as used, for example, for electrophoresis), the block will suddenly shrink to a tiny stone-like object when the water content reaches a critical low level (15-20% water, 80-85% alcohol). I may have the % wrong; remembering an experiment in the early

[Histonet] How to process tissues submitted in acrylamide

2014-01-23 Thread Catherine Simonson
Hello Helpful Histonetters, I have some samples that were fixed in PFA, placed in acrylamide gel and then treated with iodine for microCT at another facility. The investigator now wants to follow up and get HE's on these samples. I know how to deal with the iodine, but my question is this: