If it's a cross-linked polyacrylamide gel (as used, for example, for
electrophoresis), the block will suddenly shrink to a tiny stone-like object
when the water content reaches a critical low level (15-20% water, 80-85%
alcohol). I may have the % wrong; remembering an experiment in the early
Hello Helpful Histonetters,
I have some samples that were fixed in PFA, placed in acrylamide gel and
then treated with iodine for microCT at another facility. The investigator
now wants to follow up and get HE's on these samples. I know how to deal
with the iodine, but my question is this: