We are trouble-shooting cryosectioning mouse lung tissue. Often the lung
section tears or breaks apart during sectioning. In the past, if the lung
section is proving difficult to section, we take the OCT-embedded tissue and
re-embed it back into OCT (basically put fresh OCT into the original
)QIHC
Cambridge, MA
From: Kasai, Miki (NIH/NCI) [E] kas...@mail.nih.gov
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Sent: Monday, February 27, 2012 1:34 PM
Subject: [Histonet] Hydrating Frozen Tissues embedded in OCT for Sectioning
We
From: Colleen Forster cfors...@umn.edu
To: Kim Merriam kmerriam2...@yahoo.com
Sent: Monday, February 27, 2012 1:55 PM
Subject: Re: [Histonet] Hydrating Frozen Tissues embedded in OCT for Sectioning
Yep, the sample is too cold. Rubbing your finger across even with a
glove
