Behalf Of Aprill
Watanabe
Sent: Monday, February 16, 2009 6:14 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IF on cell lines
I have a colleague who is trying to do IF on cell culture cell lines. She
is growing them on cell culture treated chamber slides and fixing with 4
tonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] IF on cell lines
Hi April,
This may not be useful to your colleague at all, but here goes: I used
to place a round glass sterile coverslip in the bottom of each well of a
24-well culture plate before seeding and then grew the cells on the
coverslip.
Hi April,
This may not be useful to your colleague at all, but here goes: I used
to place a round glass sterile coverslip in the bottom of each well of a
24-well culture plate before seeding and then grew the cells on the
coverslip. When ready the coverslip was removed (using a hyperdermic
needle w
I have a colleague who is trying to do IF on cell culture cell lines. She
is growing them on cell culture treated chamber slides and fixing with 4%
formaldehyde. Her problem is that after fixation and a few washes all the
cells are detaching. Any suggestions? I think she is going to try 4%
para
