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From: Amy Porter port...@msu.edu
To: histonet@lists.utsouthwestern.edu; Nicole Collette collet...@mail.llnl.gov
Sent: Wednesday, April 1, 2009 12:56:43 PM
Subject: Re: [Histonet] IHC, decalcification preferences
All the mouse femurs and tibias done in our laboratory
Hello, all,
I am going to be doing some IHC on adult mouse bone, and since I'm
new to all this antigen preservation stuff, was wondering the best
way to decalcify? Normally, I use high percentage EDTA (17%) and
determine end point by weight loss/weight gain. (up until now have
been using
: [Histonet] IHC, decalcification preferences
Hello, all,
I am going to be doing some IHC on adult mouse bone, and since I'm new to
all this antigen preservation stuff, was wondering the best way to
decalcify? Normally, I use high percentage EDTA (17%) and determine end
point by weight loss/weight
