Hi Curt,
You can de-stain the hematoxylin counter stain and then do H and all
should be fine. Subsequent IHC is possible but obviously you would need a
different color chromogen to differentiate the new stain from the previous
one.
There may be a problem getting the section stay on through a
January 2019 4:05 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC-H HELP...
Need help before I turn a mistake into an irreparable mistake...
We have some unstained slides that were supposed to get stained with H but my
guy stained them with IHC. It's complicated, we receive
Need help before I turn a mistake into an irreparable mistake...
We have some unstained slides that were supposed to get stained with H but my
guy stained them with IHC. It's complicated, we received slides and a block,
the block was for IHC, the unstained slides were for H, he inverted the
