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Sent: Thursday, April 4, 2013 5:23 PM
Subject: [Histonet] IHC (DAB) after HE?
Dear Histonetters,
I would like to perform IHC (DAB) after HE. Do I need to remove HE
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Subject: Re: [Histonet] IHC (DAB) after HE?
Yes, you need to remove it. Hydrate your sections and place them in a 1% aq.
acetic acid solution (it will be a weak acid solution
Of MARY T HODGES
Sent: Friday, April 05, 2013 8:44 AM
To: Rene J Buesa ; nmargar...@luriechildrens.org ;
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Subject: Re: [Histonet] IHC (DAB) after HE?
I have just ihc over
Dear Histonetters,
I would like to perform IHC (DAB) after HE. Do I need to remove HE? If answer
is YES, my question HOW? Can I ask the protocol for that?
Thanks in advance,
Naira
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No. Re-hydrating the tissue will remove most (if not all) of the eosin and the
hematoxylin will serve as your counterstain (although you may need to
counterstain again at the end of you protocol). If you use antigen retrieval
for an unstained slide, you will have to use it on the HE-stained
