[Histonet] IHC Stainer Dispense volume

2013-03-21 Thread Tiana Baskin
I currently use a Dako Autostainer and we are dispensing 300uL of all reagents to the slide(100uL per drop zone). I am just curious what others are dispensing and if you've noticed any difference by changing the dispense volume in regards to stain quality. Does anyone adjust for very large

RE: [Histonet] IHC Stainer Dispense volume

2013-03-21 Thread Milne, Katy
Hi Tiana, We just got a Biocare Intellipath FLX and it came set at 300ul too. I've switched it to 150ul, dropping in the middle zone only and had no problems, may see if I can go 100ul for smaller tissues, will up it for larger tissues. I pap pen around the samples though to make sure that