RE: [Histonet] IHC antibody optimizing & validating

2014-03-25 Thread joelle weaver
y slides/tissue samples minimum for predictive markers, then you do the correlation. Keep everything for CAP. Joelle Weaver MAOM, HTL (ASCP) QIHC > From: cda...@che-east.org > To: histonet@lists.utsouthwestern.edu > Date: Tue, 25 Mar 2014 09:51:07 -0400 > Subject: [Histonet] IHC antib

[Histonet] IHC antibody optimizing & validating

2014-03-25 Thread Davis, Cassie
Will you help me? I understand we are to use the known positives controls that the manufactures' recommends in the package insert when optimizing the stains, but I need to know what is your general procedure for optimizing (how many different staining protocols do you test) and validating a new