Hi Katelin,
It would be helpful to view some images- can you post them on Histonet?
Just to be sure: You say you have intense peripheral non-specific staining and
do not see the expected immunoreactivity in the center of the tissues, or do
you see expected reactivity centrally but
mesruh
turkekul
Sent: 13 January 2010 17:57
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC edge effect
Dear Colleagues,
In my opinion the edge effect problem has many different components. In each
particular case the reason/reasons for edge effect are different.
Sometimes I observe
Dear Colleagues,
In my opinion the edge effect problem has many different components. In each
particular case the reason/reasons for edge effect are different.
Sometimes I observe that the staining intensity gradually decreases towards
the center of the section. In this case I think this is mainl