[Histonet] IHC edge effect

2010-01-13 Thread Jeffrey Silverman
  Hi Katelin,   It would be helpful to view some images- can you post them on Histonet? Just to be sure: You say you have intense peripheral non-specific staining and do not see the expected immunoreactivity in the center of the tissues, or do you see expected reactivity centrally but

RE: [Histonet] IHC edge effect

2010-01-13 Thread Dimitri Scholz
mesruh turkekul Sent: 13 January 2010 17:57 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC edge effect Dear Colleagues, In my opinion the edge effect problem has many different components. In each particular case the reason/reasons for edge effect are different. Sometimes I observe

[Histonet] IHC edge effect

2010-01-13 Thread mesruh turkekul
Dear Colleagues, In my opinion the edge effect problem has many different components. In each particular case the reason/reasons for edge effect are different. Sometimes I observe that the staining intensity gradually decreases towards the center of the section. In this case I think this is mainl