Hi Kim. You can do HIER on frozen sections, but first you must fix the sections. The purpose of HIER is to 'unmask' antigens that have been altered by the effects of fixation. We have had better results with many antibodies by fixing the frozen sections in 10% buffered formalin (30'-60'), then doing a gentle heat retrieval (~70C) or using a mild enzyme. Of course, there are times when it is preferable to use the frozen sections as is & determine if the antibody will recognize the epitope in this 'native' state. Just my experience.
Patti Loykasek BS, HTL, QIHC Clinical Lab Supervisor PhenoPath Laboratories Seattle, WA This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
