Amplification is the only way.
Possibly, tyramide amplification will help.
It will cost you to buy a kit...sure, make your own butyou have to weigh up
pros/cons
Sureyou will have to play with variables.
___
Histonet mailing list
Histonet@lis
The only thing you can do is to prolong the Ab staining time but, in order to
avoid excessive background, dilute it. You will have to find an adequate
balance between a more diluted Ab with a weaker spitope signal and a prolonged
incubation time and there is no "magic formula" for obtaining it.
Hi Everyone,
I have been doing some IHC staining on slides that are 20 years old or more.
Depending on the antibody the staining is pale in comparison to my control
slide. Does anyone have experience with staining older slides? Do you have
any tips to share? The slide storage conditions ar
